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Development of a high-performance liquid chromatography method for the determination of florfenicol in animal feedstuffs

文献类型： 外文期刊

作者： Yang, JinJing 1 ; Sun, GuiZhi 2 ; Qian, MingRong 3 ; Huang, LingLi 1 ; Ke, XianBing 2 ; Yang, Bo 2 ;

作者机构： 1.Huazhong Agr Univ, MOA Key Lab Food Safety Evaluat, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Peoples R China

2.Wuhan Inst Bioengn, Hubei Engn Res Ctr Viral Vector, 1 Hanshi Rd, Wuhan 430415, Hubei, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

关键词： High-performance liquid chromatography;Thin layer chromatography;Florfenicol;Animal feedstuffs

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

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收录情况： SCI

摘要： Highlights?A TLC-HPLC method was developed for determining florfenicol in animal feedstuffs.?The analytical method was proved to be reliable, simple and cost-effective.?TLC could be used as an alternative purification technique for complex matrix.AbstractAn effective thin layer chromatography (TLC) purification procedure coupled to high-performance liquid chromatography (HPLC) method was developed for the determination of florfenicol (FF) in pig, chicken and fish feedstuffs. The feedstuff samples were extracted with ethyl acetate, defatted with n-hexane saturated with acetonitrile, and further purified by TLC. The chromatographic separation was performed on a Waters Symmetry C18column using an isocratic procedure with acetonitrile-water (35:65, v/v) at 0.6mL/min. The ultraviolet (UV) detector was set at a wavelength of 225nm. The FF concentrations in feedstuff samples were quantified using a standard curve. Good linear correlations (y=159075x?15054,r>0.9999) were achieved within the concentration range of 0.05–200μg/mL. The recoveries of FF spiked at levels of 1, 100 and 1000μg/g ranged from 80.6% to 105.3% with the intra-day and inter-day relative standard deviation (RSD) less than 9.3%. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.02 and 0.06mg/kg for pig feedstuffs, 0.02 and 0.07mg/kg for chicken feedstuffs, and 0.02 and 0.05mg/kg for fish feedstuffs, respectively. This reliable, simple and cost-effective method could be applied to the routine monitoring of FF in animal feedstuffs.]]>

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[1]Diffusion-limited PBPK model for predicting pulmonary pharmacokinetics of florfenicol in pig. Qian, M. R.,Yang, H.,Qian, M. R.,Yang, H.,Wang, Q. Y.,Sun, G. Z.,Ke, X. B.,Yang, J. J.,Yang, B.,Huang, L. L.,Gao, J. D..

[2]Simultaneous determination of florfenicol with its metabolite based on modified quick, easy, cheap, effective, rugged, and safe sample pretreatment and evaluation of their degradation behavior in agricultural soils. Xu, Mingfei,Qian, Mingrong,Zhang, Hu,Wang, Jianmei,Wu, Huizhen,Ma, Junwei.

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