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Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris

文献类型： 外文期刊

作者： Wang, Bo 1 ; Wang, Xiuying 1 ; Tian, Yongsheng 1 ; Li, Zhenjun 1 ; Gao, Jianjie 1 ; Yan, Ying; Peng, Rihe 1 ; Yao, Qua 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China

2.Shanghai Normal Univ, Coll Life & Environm Sci, Dept Biol Sci, 100 Guili

关键词： Laccaria bicolor;laccase;Pichia pastoris;synthetic dyes

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2016 年 30 卷 1 期

页码：

收录情况： SCI

摘要： Synthetic dyes are known to be highly toxic to mammalian cells and mutagenic and carcinogenic to humans and, therefore, should be detoxified and removed from industrial effluents. Different approaches for removal and detoxication are extensively sought. Biochemical methods are considered the most economical and effective method of dye decolourization. In this research, the laccase gene from Laccaria bicolor was modified and expressed in Pichia pastoris. The properties of the recombinant laccase and its ability to degrade synthetic dyes were studied. The laccase activity was optimal at pH 2.2 and 50 degrees C. Its K-m value was 0.187 mmol/L for ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)]. The laccase obtained was shown to decolorize the synthetic dyes, malachite green, crystal violet and orange G, with ABTS as a mediator. These results indicated that the laccase obtained may be used to treat industrial effluents containing artificial dyes.

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