Isolation and Purification of a Polysaccharide from the Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes) Fruit Bodies and Its Immunomodulation of RAW 264.7 Macrophages
文献类型: 外文期刊
作者: Zhu, Lina 1 ; Tang, Qingjiu 2 ; Zhou, Shuai 2 ; Liu, Yanfang 2 ; Zhang, Zhong 2 ; Gao, Xinhua 3 ; Wang, Shiping 1 ; Wang, 1 ;
作者机构: 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
2.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai, Peoples R China
3.Shanghai Acad Agr Sci, Inst Ecoenvironm & Plant Protect, Shanghai, Peoples R China
关键词: medicinal mushrooms;Cordyceps militaris;polysaccharide;monosaccharide composition;immuno-stimulating activity
期刊名称:INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS ( 影响因子:1.921; 五年影响因子:1.879 )
ISSN: 1521-9437
年卷期: 2014 年 16 卷 3 期
页码:
收录情况: SCI
摘要: A novel polysaccharide (CP2-S) was purified from Cordyceps militaris fruit bodies by hot water extraction, ethanol precipitation, DEAE-Sepharose Fast Flow and Sephacryl S-400 high-resolution chromatography. The polysaccharide had a molecular weight of 5.938 x 10(6) g/mol and was mainly composed of glucose. CP2-S had carbohydrate content estimated to be 100% using the phenol-sulfuric acid method. Immunostimulating experiments in vitro indicated that CP2-S could stimulate nitric oxide production, phagocytosis, respiratory burst activity, and secretion of interleukin-1 beta and interleukin-2 of macrophages, suggesting that this water-soluble polysaccharide from the fruit body of C. militaris is a natural immunostimulating polysaccharide with potential for further application.
- 相关文献
作者其他论文 更多>>
-
Comprehensive evaluation of Ganoderma lucidum fermented grains: Nutritional, functional, and safety aspects
作者:Yan, Mengqiu;Jiang, Xingyi;Liu, Yanfang;Zhang, Jingsong;Feng, Jie;Yan, Mengqiu;Jiang, Xingyi;Liu, Yanfang;Zhang, Jingsong;Feng, Jie;Jiang, Xingyi
关键词:Ganoderma lucidum; Grains; Solid-state fermentation; Nutritional value; Functional efficacy; Safety
-
Nanoliposome based strategy for enhancing bioavailability and antitumor activity of polyphenol from Sanghuangporus vaninii
作者:Liu, Peng;Wang, Yuyang;Zhang, Zhong;Wu, Di;Chen, Wanchao;Li, Wen;Yang, Yan;Liu, Peng;Wang, Yuyang;Zhang, Zhong;Wu, Di;Chen, Wanchao;Li, Wen;Yang, Yan;Liu, Peng;Liu, Junbo;Wang, Weike
关键词:Sanghuangporus vaninii; Nanoliposome; Liver tumor; Polyphenol; Bioavailability
-
Cordyceps militaris extract and cordycepin ameliorate LPS-challenged colonic damage in piglets by modulating the microbiota and metabolite profiles
作者:Xiong, Shijie;Wan, Fan;Liu, Yanfang;Hang, Yiqiong;Xue, Huiqin;Lu, Yang;Xiong, Shijie;Su, Yong;Wan, Fan;Lu, Yang;Jiang, Jiajia
关键词:piglets;
Cordyceps militaris extract; cordycepin; lipopolysaccharide; colon microbiota; metabolomics -
Innovative submerged directed fermentation: Producing high molecular weight polysaccharides from Ganoderma lucidum
作者:Guo, Jia;Tang, Chenmin;Liu, Yanfang;Shi, Jia;Tang, Chuanhong;Feng, Jie;Zhang, Jingsong;Guo, Jia;Vunduk, Jovana
关键词:Ganoderma lucidum; Submerged fermentation; High molecular weight polysaccharide
-
The ENaC taste receptor's perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis
作者:Li, Wen;Chen, Wanchao;Wu, Di;Zhang, Zhong;Liu, Peng;Li, Zhengpeng;Yang, Yan
关键词:
-
Anti-hyperuricemia effects of a polysaccharide-protein complex from Lentinula edodes mediated by gut-kidney axis
作者:Xiong, Xinyi;Liu, Peng;Liu, Liping;Feng, Jie;Zhang, Jingsong;Yan, Mengqiu;Liu, Yanfang;Xiong, Xinyi;Xiong, Xinyi;Liu, Peng;Liu, Liping;Feng, Jie;Zhang, Jingsong;Yan, Mengqiu;Liu, Yanfang
关键词:Hyperuricemia; Lentinula edodes; Polysaccharide-protein complex; Gut microbiota; Gut-kidney axis
-
Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice
作者:Liu, Peng;Gao, Chen;Li, Wen;Chen, Wanchao;Zhang, Zhong;Wu, Di;Yang, Yan;Gao, Chen;Li, Tingzhao;Sun, Shuai;Li, Tingzhao
关键词:Stropharia rugosoannulata; Obesity; Protein; Hepatic steatosis; Preparation strategy



