Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus
文献类型: 外文期刊
作者: Zhang, Guo-Rui 2 ; Yu, Rui-Song 1 ; Zeng, Jiang-Yong 4 ; Zhu, Yu-Min 1 ; Dong, Shi-Juan 1 ; Dunzhu, Luobu; Zhu, Se; 1 ;
作者机构: 1.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai, Peoples R China
2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China
3.Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China
4.Tibet Acad Agr & Anim Sci, Tibet Livestock Res Inst, Lhasa, Peoples R
关键词: Competitive ELISA;Peste des petits ruminants virus;N gene;Epitope;Antibody
期刊名称:INTERVIROLOGY ( 影响因子:1.763; 五年影响因子:1.929 )
ISSN: 0300-5526
年卷期: 2013 年 56 卷 1 期
页码:
收录情况: SCI
摘要: Aims: To develop an effective diagnostic kit, based on a competitive ELISA-based system (cELISA), for detecting serum antibody against peste des petits ruminants virus (PPRV). Methods: Epitope peptides of the nucleocapsid (N) protein of Tibetan PPRV were synthesized chemically and injected into rabbits to prepare hyperimmune antisera. Test sera were incubated simultaneously with hyperimmune antisera and added to the wells of ELISA plates coated previously with recombinant N protein. Horseradish peroxidase-conjugated goat anti-rabbit antibody was employed to detect the quantity of hyperimmune antisera combined with recombinant N protein. Results: A cELISA has been developed for monitoring PPRV infections with a cutoff value of 35. Relative sensitivity and specificity values of the epitope-based cELISA were 96.18 and 91.29%, respectively, when compared with a commercial cELISA kit in a test involving 1,039 serum samples. Conclusion: We report an efficient method for preparing antibody suitable for incorporation into a cELISA that can be used routinely for the detection of PPRV antibodies in serum samples. The method eliminated the requirement for virus culture and monoclonal antibody preparation, reduced the biorisk posed by virus-dependent manipulations, and the performance of the resultant cELISA compared favorably with a commercially available cELISA kit. Copyright (c) 2012 S. Karger AG, Basel
- 相关文献
作者其他论文 更多>>
-
Coronavirus accessory protein ORF3 biology and its contribution to viral behavior and pathogenesis
作者:Si, Fusheng;Yu, Ruisong;Li, Zhen;Song, Shuai;Song, Shuai;Wei, Wenqiang;Wu, Chao
关键词:
-
Magnolol, a Neolignan-like Drug, Inhibits Porcine Epidemic Diarrhea Virus Replication in Cultured Cells
作者:Wang, Xiaoting;Zhang, Daojing;Chen, Bingqing;Yu, Ruisong;Si, Fusheng;Xie, Chunfang;Li, Zhen;Dong, Shijuan
关键词:porcine epidemic diarrhea virus (PEDV); magnolol; antiviral effect; in vitro
-
Bis-Benzylisoquinoline Alkaloids Inhibit Porcine Epidemic Diarrhea Virus In Vitro and In Vivo
作者:Dong, Shijuan;Yu, Ruisong;Chen, Bingqing;Si, Fusheng;Xie, Chunfang;Li, Zhen;Wang, Xiaoting;Zhang, Daojing;Zhou, Jiaming
关键词:PEDV; bis-benzylisoquinoline alkaloids; cepharanthine; tetrandrine; fangchinoline; antiviral effect; in vitro; in vivo
-
Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping
作者:Yu, Ruisong;Dong, Shijuan;Chen, Bingqing;Liu, Yingjie;Li, Fengping;Si, Fusheng;Xie, Chunfang;Li, Zhen;Liu, Yingjie;Li, Fengping
关键词:porcine epidemic diarrhea virus; spike protein; epitope mapping; antigenic alteration
-
Study on the Characteristic Codon Usage Pattern in Porcine Epidemic Diarrhea Virus Genomes and Its Host Adaptation Phenotype
作者:Si, Fusheng;Yu, Ruisong;Li, Zhen;Jiang, Li;Wei, Wenqiang
关键词:coronavirus; PEDV; codon usage; selection pressure; host adaptation; viral evolution
-
Identification of cellular proteins interacting with PEDV M protein through APEX2 labeling
作者:Dong, Shijuan;Wang, Ruiyang;Yu, Ruisong;Chen, Bingqing;Si, Fusheng;Xie, Chunfang;Li, Zhen;Dong, Shijuan;Chen, Bingqing;Si, Fusheng;Li, Zhen;Li, Zhen
关键词:PEDV; Membrane protein; Cellular proteins; APEX2; Interaction
-
Identification of host cell proteins that interact with the M protein of porcine epidemic diarrhea virus
作者:Wang, Ruiyang;Yu, Ruisong;Chen, Bingqing;Si, Fusheng;Wang, Jian;Xie, Chunfang;Men, Chengfang;Dong, Shijuan;Li, Zhen
关键词:Porcine epidemic diarrhea virus (PEDV); Membrane (M) protein; Interaction; Immunoprecipitation