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Improvement of Porcine Interferon-alpha Production by Recombinant Pichia pastoris via Induction at Low Methanol Concentration and Low Temperature

文献类型: 外文期刊

作者: Jin, Hu 1 ; Liu, Guoqiang 1 ; Dai, Keke 1 ; Wang, Huihui 1 ; Li, Zhen 2 ; Shi, Zhongping 1 ;

作者机构: 1.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Peoples R China

2.Shanghai Acad Agr Sci, Anim Husb & Vet Res Inst, Shanghai Municipal Key Lab Agrigenet & Breeding, Shanghai 201106, Peoples R China

关键词: Pichia pastoris;Fed-batch cultivation;Induction;Metabolic flux;Porcine interferon-alpha

期刊名称:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY ( 影响因子:2.926; 五年影响因子:2.685 )

ISSN: 0273-2289

年卷期: 2011 年 165 卷 2 期

页码:

收录情况: SCI

摘要: Improved porcine interferon-alpha (pIFN-alpha) production by recombinant Pichia pastoris was achieved by culture conditions optimization in a 5-l bioreactor. The results indicated that the pIFN-alpha concentration, specific methanol consumption rate, specific activities of alcohol oxidase, formaldehyde dehydrogenase, and formate dehydrogenase could be significantly enhanced by decreasing induction temperature. The highest pIFN-alpha concentration (1.35 g l(-1)) was obtained by simultaneously controlling methanol concentration at 5 g l(-1) and induction temperature at 20 A degrees C, which was about 1.6-fold higher than the maximum obtained with previous optimal methanol concentration level (about 10 g l(-1)) when inducing at 30 A degrees C. The potential mechanisms behind low temperature and low methanol concentration effect on pIFN-alpha production may be ascribed to higher cell metabolic activity, more carbon flux towards pIFN-alpha production, and less intracellular/extracellular protease release.

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