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Improvement of ATP regeneration efficiency and operation stability in porcine interferon-alpha production by Pichia pastoris under lower induction temperature

文献类型: 外文期刊

作者: Gao, Minjie 1 ; Dong, Shijuan 2 ; Yu, Ruisong 2 ; Wu, Jianrong 1 ; Zheng, Zhiyong 1 ; Shi, Zhongping 1 ; Zhan, Xiaobei 1 ;

作者机构: 1.Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China

2.Shanghai Acad Agr Sci, Shanghai Municipal Key Lab Agri Genet & Breeding, Anim Husb & Vet Res Inst, Shanghai 201106, Peoples R China

关键词: Fed-batch Culture;Fermentation;Pichia pastoris;Porcine Interferon-alpha;Protein Expression

期刊名称:KOREAN JOURNAL OF CHEMICAL ENGINEERING ( 影响因子:3.309; 五年影响因子:2.634 )

ISSN: 0256-1115

年卷期: 2011 年 28 卷 6 期

页码:

收录情况: SCI

摘要: The performance of traditional heterologous protein production by Pichia pastoris with methanol induction at 30 A degrees C is poor, characterized by low ATP regeneration rate and weak operation stability. A low temperature induction strategy at 20 A degrees C was thus adopted for efficient porcine interferon-alpha production in a 10 L fermentor. With the strategy, maximal methanol tolerance level could reach about 40 g/L to effectively deal with methanol concentration variations, so that the complicated on-line methanol measurement system could be eliminated. Moreover, metabolic analysis based on multiple state-variables measurements indicated that pIFN-alpha antiviral activity enhancement profited from the formation of an efficient ATP regeneration system at 20 A degrees C induction. Compared to the induction strategy at 30 A degrees C, the proposed strategy increased the ATP regeneration rate by 49-66%, the maximal pIFN-alpha antiviral activity was enhanced about 20-fold and reached a higher level of 1.5x10(6) IU/mL.

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