您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Full genomic sequence analysis of swine genotype 3 hepatitis E virus isolated from Shanghai

文献类型： 外文期刊

作者： Si, Fu-sheng 1 ; Zhu, Yu-min 1 ; Dong, Shi-juan 1 ; Yu, Shui-sheng 1 ; Yu, Rui-song 1 ; Shen, Shi-yuan 1 ; Yang, Qian; 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China

2.Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

3.Nanjing Agr

关键词： Hepatitis E virus;Genotype 3;Full genomic sequence;Phylogenetic analysis;Swine viral isolates

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2009 年 144 卷 1-2 期

页码：

收录情况： SCI

摘要： The full genomic nucleotide sequence of a previously identified genotype 3 hepatitis E virus (HEV), strain SAAS-JDY5, was obtained using RT-PCR and rapid amplification of cDNA ends (RACE). The genome consisted of 7225 nucleotides, excluding a poly-A tail at the 3' terminus, and contained three open reading frames (ORFs), ORF-1, ORF-2 and ORF-3, encoding 1702, 660 and 113 amino acids, respectively. Phylogenetic analysis confirmed that SAAS-JDY5 belonged to genotype 3 HEV and was most closely related to the Japanese isolate wbJYG1 (AB222184). SAAS-JDY5 shared approximately 87% nucleotide similarity to human and swine strains from the United States, compared with 74-75% similarity to Asian (genotype 4) and Mexican strains (genotype 2). Alignment of the SAAS-JDY5 genomic sequence with reference sequences of the same genotype revealed one nucleotide substitution and one deletion at positions 5145 and 7189 (3' UTR), respectively. Moreover, SAAS-JDY5 contained two additional nucleotides (AC) at the very end of the T-terminus preceding the poly-A tail of the genome. Comparison of the putative amino acid sequence encoded by the SAAS-JDY5 genome with sequences of other genotype 3 isolates revealed 15 unique amino acid substitutions and one deletion in ORF-1, and three substitutions in ORF-2. (C) 2009 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Molecular detection of hepatitis E virus in sheep from southern Xinjiang, China. Wu, Junyuan,Jin, Meilin,Wu, Junyuan,Jiang, Chunyu,Li, Tao,Si, Fusheng.

[2]Molecular epidemiology of hepatitis E virus infections in Shanghai, China. Zhu, Yumin,Si, Fusheng,Yu, Xiaoming,Yu, Ruisong,Dong, Shijuan,Huang, Fenfen,Li, Zhen,Zhu, Yumin,Yu, Ruisong,Dong, Shijuan,Si, Fusheng,Yu, Xiaoming,Zhang, Yuanshu,Cao, Dianjun,Huang, Fenfen. 2011

[3]Determination of the full-genome sequence of hepatitis E virus (HEV) SAAS-FX17 and use as a reference to identify putative HEV genotype 4 virulence determinants. Zhu, Yumin,Yu, Ruisong,Dong, Shijuan,Si, Fusheng,Li, Zhen,Zhu, Yumin,Yu, Ruisong,Dong, Shijuan,Si, Fusheng,Li, Zhen,Yu, Xiaoming,Zhang, Yuanshu,Huang, Fenfen. 2012

[4]Further evidence for infection of pigs with human-like H1N1 influenza viruses in China. Yu, Hai,Zhou, Yan-Jun,Li, Guo-Xin,Yan, Li-Ping,Tong, Guang-Zhi,Zhang, Gui-Hong,Liao, Ming,Liu, Hui-Li. 2009

[5]Isolation and genetic characterization of avian-like H1N1 and novel ressortant H1N2 influenza viruses from pigs in China. Zhang, Peng-Chao,Pan, Jie,Shi, Xiao-Xiao,Liu, Hui-Li,Yu, Hai,Zhou, Yan-Jun,Li, Guo-Xin,Yan, Li-Ping,Tong, Guang-Zhi. 2009

[6]Genome-Wide Identification and Analysis of the MYB Transcription Factor Superfamily in Solanum lycopersicum. Li, Zhenjun,Peng, Rihe,Tian, Yongsheng,Han, Hongjuan,Xu, Jing,Yao, Quanhong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台