ElNFS1, a nitroreductase gene from Enterobacter ludwigii, confers enhanced detoxification and phytoremediation of 4-nitrobenzaldehyde in rice
文献类型： 外文期刊
作者： Li, Zhenjun 1 ; Gao, Jianjie 1 ; Tian, Yongsheng 1 ; Wang, Bo 1 ; Xu, Jing 1 ; Fu, Xiaoyan 1 ; Han, Hongjuan 1 ; Wang, Lijuan 1 ; Zhang, Wenhui 1 ; Wang, Yu 1 ; Deng, Yongdong 1 ; Gong, Zehao 1 ; Peng, Rihe 1 ; Yao, Quanhong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China
关键词： 4-Nitrobenzaldehyde; Nitroreductase; Photosynthetic apparatus; Phytoremediation; ROS
期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：9.988； 五年影响因子：10.366 ）
ISSN： 0269-7491
年卷期： 2022 年 314 卷
页码：
收录情况： SCI
摘要： 4-nitrobenzaldehyde (4-NBA) is a widely used chemical intermediate for industrial application and an important photodegradation product of chloramphenicol. This compound represents a substantial threat to human health and ecosystem due to its genotoxic and mutagenic effect. In this study, the 4-NBA detoxification by transgenic rice overexpressing a bacterial nitroreductase gene, ElNFS1, from Enterobacter ludwigii were investigated. The cytosol-targeted ElNFS1 transgenic plants were selected to comprehensively examine their physio-biochemical responses and phytoremediation potential to 4-NBA. Our results showed that the transgenic plants exhibited strong tolerance to 4-NBA. Overexpression of ElNFS1 could significantly alleviate 4-NBA-induced damages of photosynthetic apparatus and reactive oxygen species overproduction in transgenic plants. The phytor-emediation assay revealed that transgenic plants could remove more 4-NBA from the medium than wild-type plants. HPLC and LC-MS assays showed that 4-aminobenzaldehyde was found in the reductive products of 4 -NBA. Altogether, the function of ElNFS1 during 4-NBA detoxification was characterized for the first time, which provides a strong theoretical support for the application potential of ElNFS1 transgenic plants on the phytoremediation of 4-NBA.
- 相关文献
作者其他论文 更多>>
-
Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot
作者：Wang, Bo;Wang, Yahui;Deng, Yuanjie;Xiong, Aisheng;Wang, Bo;Yao, Quanhong
关键词：Daucus carota L.; Betanin; Biosynthesis; Metabolic engineering; Bioforti fication
-
The complete degradation of 1,2-dichloroethane in Escherichia coli by metabolic engineering
作者：Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong;Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
关键词：Bioremediation; Engineered bacteria; Environmental pollution; Metabolic engineering; Synthetic biology
-
A dual-RPA based lateral flow strip for sensitive, on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops
作者：Wang, Jinbin;Wang, Yu;Hu, Xiuwen;Chen, Yifan;Jiang, Wei;Zeng, Haijuan;Liu, Hua;Wang, Jinbin;Chen, Yifan;Jiang, Wei;Zeng, Haijuan;Liu, Hua;Wang, Yu;Liu, Xiaofeng;Liu, Juan;Zhu, Lemei;Liu, Juan;Zhu, Lemei;Zeng, Haijuan;Liu, Hua
关键词：Genetically modified crops; On-site detection; Lateral flow test strips; Dual recombinase polymerase amplification; (RPA)
-
Ectopic expression of an Old Yellow Enzyme (OYE3) gene from Saccharomyces cerevisiae increases the tolerance and phytoremediation of 2-nitroaniline in rice
作者：Li, Zhenjun;Gao, Jianjie;Wang, Bo;Zhang, Hao;Tian, Yongsheng;Peng, Rihe;Yao, Quanhong
关键词：2-nitroaniline; Old Yellow Enzyme; Phytoremediation; Reactive oxygen species; Saccharomyces cerevisiae
-
Biosynthesis of melatonin from
l -tryptophan by an engineered microbial cell factory作者：Wang, Lijuan;Deng, Yongdong;Gao, Jianjie;Wang, Bo;Han, Hongjuan;Li, Zhenjun;Zhang, Wenhui;Wang, Yu;Fu, Xiaoyan;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing;Wang, Lijuan;Deng, Yongdong;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing
关键词：Biosynthesis; Melatonin;
l -Tryptophan (l -Trp); Escherichia coli; Metabolic engineering -
Response regulator KdpE contributes to Aeromonas dhakensis virulence
作者：Chen, Liqing;Wang, Yu;Fan, Lixia;Yang, Nuo;Zeng, Jifeng;Guo, Guiying;Li, Qian;Zheng, Jiping;Zeng, Jifeng;Zheng, Jiping;Wang, Yu;Wang, Peng;Zeng, Weixin
关键词：Aeromonas dhakensis; KdpE; Attenuated virulence; Histopathology; Zebrafish
-
Effects of Light Intensity on Growth and Quality of Lettuce and Spinach Cultivars in a Plant Factory
作者：Miao, Chen;Wang, Hong;Zhang, Yongxue;Cui, Jiawei;Zhang, Hongmei;Jin, Haijun;Lu, Panling;He, Lizhong;Zhou, Qiang;Yu, Jizhu;Ding, Xiaotao;Yang, Shaojun;Xu, Jing
关键词：indoor cultivation; LED light intensity; plant development; photosynthesis; nutrient content; tipburn
