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ElNFS1, a nitroreductase gene from Enterobacter ludwigii, confers enhanced detoxification and phytoremediation of 4-nitrobenzaldehyde in rice

文献类型： 外文期刊

作者： Li, Zhenjun 1 ; Gao, Jianjie 1 ; Tian, Yongsheng 1 ; Wang, Bo 1 ; Xu, Jing 1 ; Fu, Xiaoyan 1 ; Han, Hongjuan 1 ; Wang, Lijuan 1 ; Zhang, Wenhui 1 ; Wang, Yu 1 ; Deng, Yongdong 1 ; Gong, Zehao 1 ; Peng, Rihe 1 ; Yao, Quanhong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China

关键词： 4-Nitrobenzaldehyde; Nitroreductase; Photosynthetic apparatus; Phytoremediation; ROS

期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：9.988； 五年影响因子：10.366 ）

ISSN： 0269-7491

年卷期： 2022 年 314 卷

页码：

收录情况： SCI

摘要： 4-nitrobenzaldehyde (4-NBA) is a widely used chemical intermediate for industrial application and an important photodegradation product of chloramphenicol. This compound represents a substantial threat to human health and ecosystem due to its genotoxic and mutagenic effect. In this study, the 4-NBA detoxification by transgenic rice overexpressing a bacterial nitroreductase gene, ElNFS1, from Enterobacter ludwigii were investigated. The cytosol-targeted ElNFS1 transgenic plants were selected to comprehensively examine their physio-biochemical responses and phytoremediation potential to 4-NBA. Our results showed that the transgenic plants exhibited strong tolerance to 4-NBA. Overexpression of ElNFS1 could significantly alleviate 4-NBA-induced damages of photosynthetic apparatus and reactive oxygen species overproduction in transgenic plants. The phytor-emediation assay revealed that transgenic plants could remove more 4-NBA from the medium than wild-type plants. HPLC and LC-MS assays showed that 4-aminobenzaldehyde was found in the reductive products of 4 -NBA. Altogether, the function of ElNFS1 during 4-NBA detoxification was characterized for the first time, which provides a strong theoretical support for the application potential of ElNFS1 transgenic plants on the phytoremediation of 4-NBA.

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