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Porcine uterine luminal fluid-derived extracellular vesicles improve conceptus-endometrial interaction during implantation

文献类型： 外文期刊

作者： Hu, Qun 1 ; Zang, Xupeng 1 ; Ding, Yue 1 ; Gu, Ting 1 ; Shi, Junsong 1 ; Li, Zicong 1 ; Cai, Gengyuan 1 ; Liu, Dewu 1 ; Wu, Zhenfang 1 ; Hong, Linjun 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China

2.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Peoples R China

3.Lingnan Guangdong Lab Modern Agr, Guangzhou, Peoples R China

4.Guangdong Wens Breeding Swine Technol Co Ltd, Yunfu, Peoples R China

5.State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Peoples R China

关键词： Extracellular vesicles; Implantation; miRNAs; Pig; Uterine luminal fluid

期刊名称：THERIOGENOLOGY （ 影响因子：2.923； 五年影响因子：2.843 ）

ISSN： 0093-691X

年卷期： 2022 年 178 卷

页码：

收录情况： SCI

摘要： Successful implantation of porcine conceptus requires synergistic interaction with various signal molecules in the maternal uterus. Extracellular vesicles (EVs) in uterine luminal fluid (ULF) of mice play important roles in conceptus development. However, studies have not explored the roles of extracellular vesicles (EV) in ULF of pigs. The aim of this study was to identify characteristics, origin, and roles of ULFderived EVs on day 9 of the estrous cycle and on day 9,12 and 15 of pregnancy in pigs. Western blot, BCA assay and HE staining analysis showed increase in EVs concentration in ULF began from day 12 of pregnancy. Immunofluorescence staining and transmission electron microscopy analysis showed that EVs were mainly derived from endometrial epithelial cells. Fluorescent labeling, CCK-8 and transwell migration assays showed that these EVs were delivered to the trophoblast or parthenogenetic activation embryos to regulate proliferation and migration of trophoblast cells. A total of 305 miRNAs were identified using small RNA sequencing analysis. Functional enrichment analysis showed that miRNAs in these EVs potentially play vital regulatory functions in EV transportation or conceptus implantation. QRT-PCR analysis was used to further verify the RNA-seq data. The findings of this study provide information on the functions of porcine ULF-derived EVs and provide a reference dataset for future translational studies on porcine ULF-derived EVs. (c) 2021 Elsevier Inc. All rights reserved.

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