Comparative Proteomics Reveals that Phosphorylation of beta Carbonic Anhydrase 1 Might be Important for Adaptation to Drought Stress in Brassica napus
文献类型： 外文期刊
作者： Wang, Limin 1 ; Jin, Xiang 3 ; Li, Qingbin 4 ; Wang, Xuchu 3 ; Li, Zaiyun 2 ; Wu, Xiaoming 1 ;
作者机构： 1.Chinese Acad Agr Sci, Minist Agr, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Ctr Oil Crop Improvement, Natl Key Lab Crop Genet Improvement,Natl Ctr Crop, Wuhan 430070, Peoples R China
3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
4.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2016 年 6 卷
页码：
收录情况： SCI
摘要： Little is known about the mechanism of drought tolerance in rapeseed (Brassica napus L.). In this study, different morphological and physiological responses to drought stress were studied in three rapeseed cultivars. For the cultivar 2AF009 with high drought tolerance, comparative proteomic analyses were conducted to determine the molecular mechanism behind. Approximately 138 differentially abundant proteins (DAPs) and 1232 phosphoproteins containing 4469 phosphopeptides were identified. Furthermore, 337 phosphoproteins containing 547 phosphorylation sites demonstrated significant changes. These drought-responsive DAPs and phosphoproteins were mainly involved in signal transduction, photosynthesis, and glutathione-ascorbate metabolism. Notably, 9 DAPs were also identified as drought-responsive phosphoproteins, especially beta carbonic anhydrase 1 (beta CA1), which was represented by eight distinct protein spots with different abundant levels during drought stress. Tyr207 phosphorylated site of beta CA1 was down-regulated at the phosphorylation level during drought stress, which was also located in the substrate-binding active region of three-dimensional (3D) structure. Moreover, drought stress inhibited CA activity. We concluded that Tyr207 was the most likely phosphorylation target affecting the enzyme activity, and phosphorylation of beta CA1 might be important for the response to drought stress in rapeseed. The study provided a new clue for the drought tolerance mechanism in B. napus.
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