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Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model

文献类型： 外文期刊

作者： Cao, Yongsheng 1 ; Zhang, Qiya 2 ; Xu, Liming 1 ; Li, Shaowu 1 ; Wang, Di 1 ; Zhao, Jingzhuang 1 ; Liu, Hongbai 1 ; Feng, 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fishery Res Inst, Harbin, Heilongjiang, Peoples R China

2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China

3.Benxi Agrimarine Co Ltd, Benxi, Peoples R China

关键词： rainbow trout;cytokines;adjuvant;DNA vaccine;protection;Immune response;Immunology

期刊名称：ONCOTARGET （ 影响因子：5.168； 五年影响因子：5.312 ）

ISSN： 1949-2553

年卷期： 2017 年 8 卷 68 期

页码：

收录情况： SCI

摘要： Seven rainbow trout cytokine genes (interleukin (IL)-2, IL-8, IL-15, IL-17, IL-1 beta, intracellular interferon (iIFN) 1a, and IFN-gamma 2) were evaluated for their adjuvant effects on a DNA vaccine, called pG, containing the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV). Distinct DNA constructs in expression plasmid pcDNA3.1 encoding a cytokine gene were generated. Immunofluorescence assays in rainbow trout gonadal cells demonstrated successful protein expression from all these constructs. Subsequently, fish were immunized with pG alone or together with a cytokine expression plasmid. Results showed that each cytokine plasmids at an appropriate dose showed notable effects on immune gene expression. IL-17 and IFN-gamma 2 can enhance early specific IgM response. All cytokines, except IL-8, can benefit initial neutralizing antibody (NAb) titers. At 35 days post immunization (dpi), NAb titers of fish immunized with pG and IL-2, iIFN1a, or IFN-gamma 2 plasmids remained at high levels (1: 160). NAb titers of fish immunized with pG alone decreased to 1: 40. IL-8 or IL-1 beta can enhance antigen-specific proliferative T-cell responses at 14 dpi. At 28 dpi, coinjection of pG with IL-2, IL-8, IL-15, or IL-17 plasmids induced considerably stronger lymphocyte proliferation than that with injection of pG alone. All cytokine plasmids delivered with pG plasmid enhanced protection of trout against IHNV-mediated mortality. These results indicate that the type and dose of trout cytokine genes injected into fish affect quality of immune response to DNA vaccination.

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