Establishment and characterization of a gill cell line from turbot (Scophthalmus maximus)
文献类型： 外文期刊
作者： Wang, Jiaqi 1 ; Shi, Xiang 3 ; Gong, Zhihong 1 ; Chen, Songlin 4 ; Hu, Guobin 1 ;
作者机构： 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
2.Ocean Univ China, Inst Evolut & Marine Biodivers, MoE Key Lab Evolut & Marine Biodivers, Qingdao 266003, Peoples R China
3.Ocean Univ China, Haide Coll, Qingdao 266100, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China
关键词： Cell culture; SMG cell line; Differentially expressed genes; Immune mechanisms
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.9； 五年影响因子：4.2 ）
ISSN： 1050-4648
年卷期： 2025 年 161 卷
页码：
收录情况： SCI
摘要： Turbot (Scophthalmus maximus) is a main breeding marine fish species in northern China, but its aquaculture industry is seriously threatened by bacterial diseases. The turbot cell line will provide effective tools for the study of turbot immunity and basic biology. In this study, a continuous passaged Scophthalmus maximus gill (SMG) cell line was established through primary culture and subculture, and the optimal culture conditions were determined. The culture medium consists only of a basal medium L-15, fetal bovine serum (FBS) and antibiotics without supplementing growth factors and other additives. Its turbot-derivation was verified by chromosomal analysis and 18S rRNA sequencing. We also confirmed that pEGFP-C1 plasmid could transfect SMG cells. After lipopolysaccharide (LPS) stimulation, the transcriptome of SMG cells changed. The results of gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis showed that the differentially expressed genes were significantly enriched in immune-related signaling pathways such as Toll-like receptor signaling pathway and RIG-I-like receptor signaling pathway. Taken together, we established an easy-to-culture cell line from turbot gill, which provides a convenient tool for studying turbot diseases and immune mechanisms.
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