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Gdnf Acts as a Germ Cell-Derived Growth Factor and Regulates the Zebrafish Germ Stem Cell Niche in Autocrine- and Paracrine-Dependent Manners

文献类型： 外文期刊

作者： Doretto, Lucas B. 1 ; Butzge, Arno J. 1 ; Nakajima, Rafael T. 1 ; Martinez, Emanuel R. M. 1 ; de Souza, Beatriz Marques 1 ; Rodrigues, Maira da Silva 1 ; Rosa, Ivana F. 1 ; Ricci, Juliana M. B. 1 ; Tovo-Neto, Aldo 1 ; Costa, Daniel F. 1 ; Malafaia, Guilherme 1 ; Shao, Changwei 3 ; Nobrega, Rafael H. 1 ;

作者机构： 1.Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Reprod & Mol Biol Grp, BR-18618689 Sao Paulo, Brazil

2.Goiano Fed Inst, Biol Res Lab, Urata Campus,Rodovia Geraldo Silva Nascimento, BR-75790000 Urutai, Brazil

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao 266072, Peoples R China

4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China

关键词： Gdnf; Gfr alpha 1; spermatogonial stem cell; spermatogenesis; zebrafish

期刊名称：CELLS （ 影响因子：7.666； 五年影响因子：7.677 ）

ISSN：

年卷期： 2022 年 11 卷 8 期

页码：

收录情况： SCI

摘要： Glial cell line-derived neurotrophic factor (GDNF) and its receptor (GDNF Family Receptor alpha 1-GFR alpha 1) are well known to mediate spermatogonial stem cell (SSC) proliferation and survival in mammalian testes. In nonmammalian species, Gdnf and Gfr alpha 1 orthologs have been found but their functions remain poorly investigated in the testes. Considering this background, this study aimed to understand the roles of the Gdnf-Gfr alpha 1 signaling pathway in zebrafish testes by combining in vivo, in silico and ex vivo approaches. Our analysis showed that zebrafish exhibit two paralogs for Gndf (gdnfa and gdnfb) and its receptor, Gfr alpha 1 (gfr alpha 1a and gfr alpha 1b), in accordance with a teleost-specific third round of whole genome duplication. Expression analysis further revealed that both ligands and receptors were expressed in zebrafish adult testes. Subsequently, we demonstrated that gdnfa is expressed in the germ cells, while Gfr alpha 1a/Gfr alpha 1b was detected in early spermatogonia (mainly in types A(und) and A(diff)) and Sertoli cells. Functional ex vivo analysis showed that Gdnf promoted the creation of new available niches by stimulating the proliferation of both type A(und) spermatogonia and their surrounding Sertoli cells but without changing pou5f3 mRNA levels. Strikingly, Gdnf also inhibited late spermatogonial differentiation, as shown by the decrease in type B spermatogonia and down-regulation of dazl in a co-treatment with Fsh. Altogether, our data revealed that a germ cell-derived factor is involved in maintaining germ cell sternness through the creation of new available niches, supporting the development of spermatogonial cysts and inhibiting late spermatogonial differentiation in autocrine- and paracrine-dependent manners.

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