文献类型： 外文期刊

作者： Wang, Cheng-Zhen ¹ ; Gao, Xun ¹ ; Tu, Jie-Ying ¹ ; Lv, Lu-Chao ¹ ; Pu, Wen-Xian ¹ ; He, Xiao-Tong ¹ ; Jiao, Yan-Xiang ¹ ; Deng, Yu-Ting ⁵ ; Liu, Jian-Hua ¹ ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Guangdong Prov Key Lab Vet Pharmaceut Dev & Safety, Guangzhou, Peoples R China

2.South China Agr Univ, Coll Vet Med, Key Lab Zoonosis, Minist Agr & Rural Affairs, Guangzhou, Peoples R China

3.South China Agr Univ, Coll Vet Med, Natl Risk Assessment Lab Antimicrobial Resistance, Guangzhou, Peoples R China

4.Guangdong Lab Lingnan Modern Agr, Guangzhou, Peoples R China

5.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr, Guangzhou, Peoples R China

6.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol, Guangzhou, Peoples R China

关键词： tigecycline resistance; Aeromonas; efflux pump; 2-toprJ2; mobile genetic element

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：9.043； 五年影响因子：8.113 ）

ISSN： 2165-0497

年卷期：

页码：

收录情况： SCI

摘要： The appearance and prevalence of novel plasmid-encoded tigecycline resistance efflux pump gene clusters tmexC1D1-toprJ1 and tmexC2D2-toprJ2 in Enterobacteriaceae have raised a threat to public health. Here, another tigecycline resistance gene cluster, tmexC2D2.2-toprJ2, was identified in two Aeromonas isolates recovered from fish meat and vegetables. Cloning confirmed the expression of tmexC2D2.2-toprJ2 mediated the resistance to tigecycline and decreased susceptibility to tetracyclines and cephalosporins in both Escherichia coli and Aeromonas. In an Aeromonas veronii strain, four copies of tmexC2D2.2-toprJ2 were located on the chromosome. Further analysis revealed that tmexC2D2.2-toprJ2 has been detected in the chromosomes of A. veronii, Aeromonas hydrophila, and Aeromonas caviae with one to four copies due to the insertion of a potential integrative transferable unit. The occurrence of multiple copies of chromosomal tmexC2D2.2-toprJ2 may act as a sink for this tigecycline resistance gene cluster, which requires continuous monitoring. IMPORTANCE Tigecycline is regarded as one of the few effective drugs against multidrug-resistant bacterial infection. However, mobile tigecycline resistance efflux pump gene clusters such as tmexC1D1-toprJ1 and its variants have been identified in both animal- and human-origin Enterobacteriaceae. In this study, we first found another efflux pump gene cluster, tmexC2D2.2-toprJ2, in the Aeromonas chromosome. This gene cluster could mediate tigecycline resistance and decrease susceptibility to tetracyclines and cephalosporins in the Aeromonas host strain. Meanwhile, tmexC2D2.2-toprJ2 was detected with multiple copies in Aeromonas spp. This multidrug resistance efflux pump gene cluster with multiple copy numbers might stably exist in Aeromonas and serve as a reservoir for tmexCD2-toprJ2, facilitating its persistent presence and spread.