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Nuclear Factor-?B Plays a Positive Role in TNFa Expression in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

文献类型： 外文期刊

作者： Zhu, Ke-Cheng 1 ; Guo, Hua-Yang 1 ; Guo, Liang 1 ; Liu, Bao-Suo 1 ; Zhang, Nan 1 ; Jiang, Shi-Gui 1 ; Zhang, Dian-Chang 1 ;

作者机构： 1.Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Exploita, Guangzhou, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Peoples R China

3.Guangdong Prov Engineer Technol Res Ctr Marine Bio, Guangzhou, Peoples R China

4.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Trop Aquaculture Res & Dev Ctr, Sanya, Peoples R China

5.Sanya Trop Fisheries Res Inst, Sanya, Peoples R China

关键词： Trachinotus ovatus; promoter activity; transcription factor; TNF alpha; NF-kappa B

期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：5.247； 五年影响因子：5.72 ）

ISSN：

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： Tumour necrosis factor-alpha (TNF alpha) is a multifarious mediator of lymphoid tissue growth and antimicrobial defence mechanisms, and it acts as a pro-inflammatory regulator. The function of TNF alpha in parasite infection and the underlying mechanism through which nuclear factor-kappa B (NF-kappa B) regulates TNF alpha remain largely unclear in teleosts. In the present study, TNF alpha (ToTNF alpha) from golden pompano (Trachinotus ovatus) was identified, and its sequence features and expression levels were determined. The genomic DNA sequence is composed of 1,130 bp, consists of four exons and three introns, and encodes 341 amino acid polypeptides. The putative protein sequence shares 34.7%-61.9% identity with fish TNF alpha and possesses a TNF family signature and two conserved cysteine residues. Moreover, the expressions of ToNF-kappa B and ToTNF alpha are constitutively expressed in all examined tissues, with higher levels observed in the immune relevant tissues. Both ToNF-kappa B and ToTNF alpha transcription was increased in the local infection sites (skin and gill) and system immune tissues (liver, spleen and head kidney) after Cryptocaryon irritans stimulation. In addition, to investigate whether ToNF-kappa B is a regulator of ToTNF alpha, promoter analysis was performed. The region from -970 to +79 bp is known as the core promoter by different truncated mutants of ToTNF alpha. Subsequently, the activity of the ToTNF alpha-p2 promoter was dramatically reduced after targeted mutation of the M6-binding site. Additionally, an electrophoretic mobile shift assay (EMSA) verified that ToNF-kappa B interacted with the M6-binding site in the ToTNF alpha promoter region to control the expression of ToTNF alpha. In conclusion, the present study provides the positive regulation of TNF alpha transcription by NF-kappa B and contributes to a better understanding of the transcriptional mechanism of TNF alpha in fish.

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