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Effects of Quorum Sensing AHL Signaling on the Biological Characteristics of Porcine Derived F4ac+Enterotoxigenic Escherichia coli

文献类型： 外文期刊

作者： Yang, Yang 1 ; Shao, Ji 1 ; Zhou, Mingxu 3 ; Duan, Qiangde 1 ; Zhang, Xinyi 1 ; Zhu, Guoqiang 1 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China

2.Jiangsu Coinnovat Ctr Important Anim Infect Dis, Yangzhou 225009, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Natl Res Ctr Engn & Technol Vet Biol, Nanjing 210014, Peoples R China

4.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety State Key Lab, Nanjing 210014, Peoples R China

关键词： ETEC; Quorum sensing; Acyl-homoserine-lactone; Virulence factor; 3OC12-HSL and C6-HSL; AHL signals

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.6； 五年影响因子：0.7 ）

ISSN： 0030-9923

年卷期： 2022 年 54 卷 2 期

页码：

收录情况： SCI

摘要： Diarrhea of piglets is an important disease affecting the development of pig industry, while F4 enterotoxigenic Escherichia coli (ETEC) is an important pathogen causing diarrhea of newborn or weaned piglets, which seriously harms animal husbandry and causes huge economic losses. The pathogenicity of E. coli is closely related to its virulence factors, which are strictly regulated in vivo, and the quorum sensing system is involved in this process. To study the effect of quorum sensing signal molecule acyl homoserine lactone (AHL) on the biological characteristics of Enterotoxigenic E. coli (ETEC), gene lasI of Pseudomonas aeruginosa which is responsible for the synthesis of long side-chain AHL (3OC12-HSL), and gene yenI of Yersinia enterocolitica which is responsible for the synthesis of short side-chain AHL (C6-HSL), were selected and transformed into ETEC strain C83902, respectively, and endowed with the ability of endogenous synthesis of 3OC12-HSL and C6-HSL. At the same time, 200 mu mol/L 3OC12-HSL and C6-HSL were added into the culture medium, respectively, and co-cultured with ETEC strain C83902. ETEC strains were stimulated by exogenous and endogenous long-chain and short-chain AHL molecules, respectively, and their biological characteristics, such as growth characteristics, biofilm formation ability, and adhesion ability to piglets intestinal epithelial cells IPEC-J2 were observed under the influence of the AHLs described above. Real-time PCR was used to detect the transcription levels of genes encoding major subunits of pathogenic factors such as fimbriae, flagella, enterotoxin, adhesin and hemolysin under the influence of the AHLs. The results showed that under the influence of quorum sensing AHLs, the biofilm formation ability and adherence ability of ETEC were significantly reduced, and the expression of fliC and other major virulence factors were regulated. AHL signaling molecules can regulate virulence related characteristics of F4ac+ ETEC.

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