Bioassay-guided isolation and identification of active compounds from Macleaya microcarpa (Maxim) Fedde against fish pathogenic bacteria
文献类型： 外文期刊
作者： Kang, Yu-Jun 1 ; Yi, Yang-lei 1 ; Zhang, Chao 1 ; Wu, Shu-Qin 2 ; Shi, Cun-Bin 2 ; Wang, Gao-Xue 1 ;
作者机构： 1.Northwest A&F Univ, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China
关键词： alkaloids;Macleaya microcarpa;fish pathogenic bacteria;antibacterial agent
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）
ISSN： 1355-557X
年卷期： 2013 年 44 卷 8 期
页码：
收录情况： SCI
摘要： Four alkaloids (Sanguinarine, 6-Methoxyl-dihydro-chelerythrine, Cryptopine and -Allocryptopine) were isolated from aerial parts of Macleaya microcarpa (Maxim) Fedde using bioassay-guided isolation method, and the inhibitory activity of ethanolic extract, various fractions and these four alkaloids against four fish pathogenic bacteria (Aeromonas hydrophila, Aeromonas salmonicida, Vibrio anguillarum and Vibrio harveyi) was assessed in vitro using the agar dilution method and the microdilution assay method respectively. A. hydrophila was the most sensitive strain to all the tested compounds. Minimum inhibitory concentration (MIC) values were lower for sanguinarine against all tested Gram-negative strains than other three alkaloids, with MIC values of 12.5mgL(-1) for A. hydrophila and 50mgL(-1) to other pathogenic bacteria. Followed by 6-methoxyl-dihydro-chelerythrine, which showed considerable antibacterial activity with MIC values of 80mgL(-1) for A. hydrophila, 100mgL(-1) for V. harveyi, and 125mgL(-1) for both V. anguillarum and A. salmonicida. Cryptopine and -allocryptopine revealed similar inhibitory activity with MIC values of 100mgL(-1) for A. hydrophila and 200mgL(-1) for other three bacterial species. These finding provided evidence that extract, as well as isolated compounds from M. microcarpa might be potential sources novel antibacterial agents for the treatment of fish infectious diseases.
- 相关文献
作者其他论文 更多>>
-
SpCrus2 Glycine-Rich Region Contributes Largely to the Antiviral Activity of the Whole-Protein Molecule by Interacting with VP26, a WSSV Structural Protein
作者：Wang, Yue;Ma, Hong-Yu;Wang, Yue;Fang, Wen-Hong;Li, Xin-Cang;Wang, Yue;Ma, Hong-Yu;Zhang, Chao
关键词：Scylla paramamosain; crustin; glycine-rich region (GRR); cysteine-rich region (CRR); WSSV; binding and antiviral activities
-
Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)
作者：Wang, Qishuo;Chen, Jiakang;Wang, Xuepeng;Wang, Qishuo;Chen, Jiakang;Wang, Xuepeng;Wang, Qishuo;Zhang, Chao;Xu, La;Wang, Xuepeng
关键词：bacterial ghosts; challenge; immune response; Streptococcus iniae; tilapia
-
Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effect of an immersion DNA vaccine against infectious spleen and kidney necrosis virus in mandarin fish
作者：Lin, Qiang;Li, Ning-Qiu;Huang, Zhi-Bin;Fu, Xiao-Zhe;Zhao, Zhao;Zhang, Chen;Wang, Gao-Xue;Zhu, Bin;Zhao, Mi;Zhao, Mi
关键词：Infectious spleen and kidney necrosis virus; DNA vaccine; Single-walled carbon nanotubes; Mandarin fish
-
Immersion vaccination of Mandarin fish Siniperca chuatsi against infectious spleen and kidney necrosis virus with a SWCNTs-based subunit vaccine
作者：Zhao, Zhao;Zhang, Chen;Jia, Yi-Jun;Qiu, De-Kui;Wang, Gao-Xue;Zhu, Bin;Lin, Qiang;Li, Ning-Qiu;Huang, Zhi-Bin;Fu, Xiao-Zhe
关键词：Infectious spleen and kidney neocrosis virus; Siniperca chuatsi; Carbon nanotubes; Immune response; Vaccine
-
Detection and quantification of Aeromonas schubertii in Channa maculata by TaqMan MGB probe fluorescence real-time quantitative PCR
作者：Liu, Chun;Lin, Li;Liu, Chun;Cao, Jizhen Z.;Zhang, De-Feng;Chang, Ou-Qin;Li, Kaibin;Wang, Fang;Shi, Cun-Bin;Jiang, Lan;Wang, Qing;Guo, Yanming M.;Lin, Li
关键词：Aeromonas schubertii; bacterial load; Channidae; TaqMan MGB real-time PCR
-
Involvement of a newly identified atypical type II crustin (SpCrus5) in the antibacterial immunity of mud crab Scylla paramamosain
作者：Wang, Yue;Zhang, Xiao-Wen;Wang, Yue;Wang, Hui;Huang, Yan-Qing;Lu, Jian-Xue;Li, Xin-Cang;Wang, Yue;Wang, Hui;Huang, Yan-Qing;Lu, Jian-Xue;Li, Xin-Cang;Zhang, Chao;Ma, Hongyu
关键词：Crustin; Glycine-rich region; Expression profile; Antibacterial activity; Binding activity; Agglutination activity
-
Effects of three strains of intestinal autochthonous bacteria and their extracellular products on the immune response and disease resistance of common carp, Cyprinus carpio
作者：Chi, Cheng;Jiang, Bing;Yu, Xiao-Bo;Liu, Tian-Qiang;Wang, Gao-Xue;Xia, Lei
关键词：Intestinal autochthonous bacteria;Innate immunity;Disease resistance;Cyprinus carpio
