您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome-Wide Identification, Expression Profile and Evolution Analysis of Karyopherin beta Gene Family in Solanum tuberosum Group Phureja DM1-3 Reveals Its Roles in Abiotic Stresses

文献类型： 外文期刊

作者： Xu, Ya 1 ; Liu, Lu 2 ; Zhao, Pan 2 ; Tong, Jing 3 ; Zhong, Naiqin 2 ; Zhang, Hongji 1 ; Liu, Ning 2 ;

作者机构： 1.Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China

2.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

4.Enterprise Key Lab Adv Technol Potato Fertilizer, Hulunbuir 021000, Inner Mongolia, Peoples R China

关键词： karyopherin; solanum tuberosum; abiotic stress; expression analysis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 3 期

页码：

收录情况： SCI

摘要： In eukaryotic cells, nucleocytoplasmic trafficking of macromolecules is largely mediated by Karyopherin beta/Importin (KPN beta or Imp beta) nuclear transport factors, and they import and export cargo proteins or RNAs via the nuclear pores across the nuclear envelope, consequently effecting the cellular signal cascades in response to pathogen attack and environmental cues. Although achievements on understanding the roles of several KPN beta s have been obtained from model plant Arabidopsis thaliana, comprehensive analysis of potato KPN beta gene family is yet to be elucidated. In our genome-wide identifications, a total of 13 StKPN beta (Solanum tuberosum KPN beta) genes were found in the genome of the doubled monoploid S. tuberosum Group Phureja DM1-3. Sequence alignment and conserved domain analysis suggested the presence of importin-beta N-terminal domain (IBN_N, PF08310) or Exporin1-like domain (XpoI, PF08389) at N-terminus and HEAT motif at the C-terminal portion in most StKPN beta s. Phylogenetic analysis indicated that members of StKPN beta could be classified into 16 subgroups in accordance with their homology to human KPN beta s, which was also supported by exon-intron structure, consensus motifs, and domain compositions. RNA-Seq analysis and quantitative real-time PCR experiments revealed that, except StKPN beta 3d and StKPN beta 4, almost all StKPN beta s were ubiquitously expressed in all tissues analyzed, whereas transcriptional levels of several StKPN beta s were increased upon biotic/abiotic stress or phytohormone treatments, reflecting their potential roles in plant growth, development or stress responses. Furthermore, we demonstrated that silencing of StKPN beta 3a, a SA- and H2O2-inducible KPN beta genes led to increased susceptibility to environmental challenges, implying its crucial roles in plant adaption to abiotic stresses. Overall, our results provide molecular insights into StKPN beta gene family, which will serve as a strong foundation for further functional characterization and will facilitate potato breeding programs.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台