您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Histone Acetylation at the Promoter for the Transcription Factor PuWRKY31 Affects Sucrose Accumulation in Pear Fruit(1)([OPEN])

文献类型： 外文期刊

作者： Li, Xinyue 1 ; Guo, Wei 1 ; Li, Juncai 2 ; Yue, Pengtao 1 ; Bu, Haidong 1 ; Jiang, Jing 1 ; Liu, Weiting 1 ; Xu, Yaxiu 1 ; Y 1 ;

作者机构： 1.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China

2.LiaoNing Inst Pomol, Xiongyue 115009, Peoples R China

3.Heilongjiang Acad Agr Sci, Mudanjiang Branch, Mudanjiang 157000, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

ISSN： 0032-0889

年卷期： 2020 年 182 卷 4 期

页码：

收录情况： SCI

摘要： High acetylation in the promoter of a transcription factor gene is associated with sucrose accumulation in pear fruit by activating the expression of a sucrose transport gene. Sugar content is an important trait of fleshy fruit, and elevating Suc levels is a major goal in horticultural crop breeding. Here, we examined the sugar content in two varieties of the Ussurian pear (Pyrus ussuriensis), 'Nanguo' (NG) and its bud sport (BNG), and we found that Suc content was higher in BNG fruit than in NG fruit. We compared the transcriptomes of the two varieties using RNA sequencing and identified a SWEET (Sugars Will Eventually be Exported Transporter) gene, PuSWEET15, expressed at higher levels in BNG fruit. Heterologous expression of PuSWEET15 in a SUSY7/ura yeast (Saccharomyces cerevisiae) strain showed that PuSWEET15 is an active Suc transporter. Overexpression of PuSWEET15 in NG pear fruit increased Suc content, while silencing of PuSWEET15 in BNG fruit decreased Suc content. The WRKY transcription factor PuWRKY31 was also expressed more highly in BNG fruit than in NG fruit, and we found that PuWRKY31 bound to the PuSWEET15 promoter and induced its transcription. The histone acetylation level of the PuWRKY31 promoter was higher in BNG fruit, suggesting a mechanism by which Suc levels can be elevated.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台