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A highly sensitive monoclonal antibody-based paper sensor for simultaneously detecting valnemulin and tiamulin in porcine liver

文献类型： 外文期刊

作者： Na, Guanqiong 1 ; Hu, Xiaofei 2 ; Sun, Yaning 2 ; Kwee, Sharon 3 ; Xing, Guangxu 2 ; Xing, Yunrui 2 ; Zhang, Gaiping 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

3.Univ Texas San Antonio, Dept Biomed Engn, San Antonio, TX 78249 USA

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

5.Yangzhou Univ, Centerfor Prevent & Control Important Anim Infect, Yangzhou 225009, Jiangsu, Peoples R China

关键词： immunoassay strip; pleuromutilin; rapid test; tiamulin; valnemulin

期刊名称：JOURNAL OF FOOD SCIENCE （ 影响因子：3.167； 五年影响因子：3.376 ）

ISSN： 0022-1147

年卷期： 2020 年 85 卷 6 期

页码：

收录情况： SCI

摘要： Valnemulin (VAL) and tiamulin (TIA) are pleuromutilin antibiotics used primarily for treating bacterial infections in swine or other food animals. Furthermore, VAL and TIA are also employed as feed additives to promote animal growth. However, the illegal use of VAL and TIA could cause a series of hazards to consumers. Here, VAL was designed to be conjugated with bovine serum protein to prepare immunogen. A highly sensitive monoclonal antibody that recognized both VAL and TIA has been successfully produced. Moreover, an immunochromatographic strip assay for rapidly screening VAL and TIA in porcine liver was established with visual detection limits (cutoff values) of 50 and 25 ng/g, respectively. The IC50 values calculated from the equation of the standard curve were 6.06 and 3.45 ng/g and the limits of detection were 0.96 and 0.29 ng/g for VAL and TIA. According to the recovery experiment results, the test strip exhibited acceptable accuracy and precision. Generally, the proposed strip provided a practical tool for the detection of VAL and TIA. Practical Application We produced a highly sensitive monoclonal antibody and developed an immunoassay strip for simultaneously monitoring TIA and VAL. Additionally it was preliminarily confirmed that the rapid detection tool was suitable for screening TIA and VAL in porcine liver.

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