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Heterologous expression and characterization of a bilirubin oxidase gene from Myrothecium verrucaria in Arabidopsis thaliana

文献类型： 外文期刊

作者： Feng, Huijuan 1 ; Wang, Mingqing 3 ; Wang, Bo 2 ; Zhang, Ling 4 ; Zhang, Fujian 2 ; Xu, Jing 2 ; Tian, Yongsheng 2 ; Gao, 1 ;

作者机构： 1.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Biol, Shanghai, Peoples R China

2.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China

3.Shanghai Normal Univ, Coll Life Sci, Dept Biol, Shanghai, Peoples R China

4.Nanjing Agr Univ, Coll Hort, Dept Pomol, Nanjing, Peoples R China

关键词： Bilirubin oxidase; heterologous expression; arabidopsis thaliana; dye; decolorization

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2020 年 34 卷 1 期

页码：

收录情况： SCI

摘要： Bilirubin oxidases (BODs), which catalyze the oxidation of bilirubin to biliverdin, are multi-copper oxidases that can be used in clinical diagnoses. Given their different reported enzymatic characterizations from laccases, BODs have gained considerable attention for their application in healthcare and environment management. In this study, a BOD gene from Myrothecium verrucaria was chemically synthesized and optimized to heterogeneous expression in Arabidopsis thaliana. The optimized DNA sequence showed 80.1% similarity to the original sequences. The gene was successfully transferred into A. thaliana, and the crude extracts of transgenic plants showed higher BOD activity compared with that of untransformed plants. BOD expressed in transgenic A. thaliana also exhibited an activity on the typical substrate (ABTS) of laccase and was able to decolorize the textile dyes crystal violet and malachite green. The results indicated that the M. verrucaria BOD gene could be transgenically exploited in plants for dye decolorization.

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