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Clathrin-dependent endocytosis predominantly mediates protein absorption by fat body from the hemolymph in Bombyx mori

文献类型： 外文期刊

作者： Wang, Yu-Jie 1 ; Li, Shu-Yan 1 ; Zhao, Jia-Ye 1 ; Li, Kang 2 ; Xu, Jing 1 ; Xu, Xian-Ying 1 ; Wu, Wen-Mei 1 ; Yang, Rong; 1 ;

作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangdong Prov Sericulture & Mulberry Engn Res Ct, Coll Anim Sci, Guangzhou, Peoples R China

2.South China Normal Univ, Guangdong Prov Key Lab Insect Dev Biol & Appl Tec, Inst Insect Sci & Technol, Sch Life Sci, Guangzhou, Peoples R China

3.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou, Peoples R China

关键词： Bombyx mori; clathrin-mediated endocytosis; fat body; hemolymph; uptake of proteins

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2020 年 27 卷 4 期

页码：

收录情况： SCI

摘要： During insect larval-pupal metamorphosis, proteins in the hemolymph are absorbed by the fat body for the maintenance of intracellular homeostasis; however, the type of proteins and how these proteins are internalized into the fat body are unclear. In Bombyx mori, the developmental profiles of total proteins in the hemolymph and fat body showed that hemolymph-decreased protein bands (55-100 kDa) were in accordance with those protein bands that increased in the fat body. Inhibition of clathrin-dependent endocytosis predominantly blocked the transportation of 55-100 kDa proteins from the hemolymph into the fat body, which was further verified by RNA interference treatment of Bmclathrin. Six hexamerins were shown to comprise similar to 90% of the total identified proteins in both the hemolymph and fat body by mass spectrum (MS) analysis. In addition, hemolymph-specific proteins were mainly involved in material transportation, while fat body-specific proteins particularly participated in metabolism. In this paper, four hexamerins were found for the first time, and potential proteins absorbed by the fat body from the hemolymph through clathrin-dependent endocytosis were identified. This study sheds light on the protein absorption mechanism during insect metamorphosis.

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