Highly sensitive phage-magnetic-chemiluminescent enzyme immunoassay for determination of zearalenone
文献类型： 外文期刊
作者： Liu, Ruxia 1 ; Shi, Ruirui 1 ; Zou, Wenting 1 ; Chen, Wenhua 2 ; Yin, Xianchao 2 ; Zhao, Fengchun 1 ; Yang, Zhengyou 1 ;
作者机构： 1.Shandong Agr Univ, Coll Life Sci, Shandong Key Lab Agr Microbiol, Tai An 271018, Shandong, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China
关键词： Phage-display; Magnetic nanoparticles; Mimotope; Zearalenone; Chemiluminescent
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2020 年 325 卷
页码：
收录情况： SCI
摘要： Here we demonstrate a novel phage-magnetic-chemiluminescent enzyme immunoassay (P-MCLEIA) for detection of zearalenone (ZEN). The P-MCLEIA was more efficient than conventional ELISA through several improvements. In the P-MCLEIA, magnetic nanoparticles were replaced of microplates as solid phases to reduce the whole incubation time within 40 min. Phage-mimotope was replaced of chemosynthetic antigen to improve the sensitivity of immunoassay. Chemiluminescence substrate was replaced of chromogenic substrate to further improve the sensitivity. The IC50, value of P-MCLEIA was 31.4 pg/mL, which was about 11 times lower than that of phage-magnetic-enzyme linked immunosorbent assay (P-MELISA) and 72 times lower than that of conventional ELISA. The LOD of P-MCLEIA was 4.3 pg/mL. Recovery study of P-MCLEIA was performed by analyzing ZEN levels in spiked corn samples, infra- and inter-assay recoveries were 80.0-119.8% and 82.7-112.7%, respectively. Furthermore, parallel analysis of natural corn samples showed a good correlation between the P-MCLEIA and high performance liquid chromatography.
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