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Colloidal gold-based immunochromatographic strip assay for the rapid detection of bacitracin zinc in milk

文献类型： 外文期刊

作者： Na, Guanqiong 1 ; Hu, Xiaofei 2 ; Yang, Jifei 2 ; Sun, Yaning 2 ; Kwee, Sharon 3 ; Tang, Liang 3 ; Xing, Guangxu 2 ; Xing 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China

3.Univ Texas San Antonio, Dept Biomed Engn, San Antonio, TX 78249 USA

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

关键词： Bacitracin; Monoclonal antibody; Immunochromatographic strip; Rapid detection tool

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2020 年 327 卷

页码：

收录情况： SCI

摘要： In this study, a murine monoclonal antibody (mAb) of 6D2-G10 against bacitracin zinc (BAC) was produced and applied to an immunochromatographic strip (ICS) for the initial detection of BAC in milk. The ICS with a cut-off value of 25 ng/mL could be perceived by the naked eye within 10 min. With the assist of the strip reader, the limit of detection (LOD) was measured as 0.82 ng/mL, the half-maximal inhibitory concentration (IC50) was recorded as 3.16 ng/mL, and the linear detection range was from 0.97 to 10.30 ng/mL. The recoveries ranged from 87.7% to 96.0% with the highest coefficient of variation (CV) of 9.1% in the intra-assay and from 84.3% to 90.2% with the highest CV of 10.7% in the inter-assay. In short, the established ICS provided a serviceable analytical tool for qualitatively and quantitatively monitoring BAC in milk.

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