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The Expression of microRNA in Adult Rat Heart with Isoproterenol-Induced Cardiac Hypertrophy

文献类型： 外文期刊

作者： Gan, Mailin 1 ; Zhang, Shunhua 1 ; Fan, Yuan 1 ; Tan, Ya 1 ; Guo, Zhixian 1 ; Chen, Lei 1 ; Bai, Lin 1 ; Jiang, Dongmei 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu 611130, Peoples R China

2.Sichuan Agr Univ, Farm Anim Genet Resource Explorat & Innovat Key L, Chengdu 611130, Peoples R China

3.Guizhou Acad Agr Sci, Inst Anim Husb & Vet, Guiyang 550005, Peoples R China

关键词： cardiac hypertrophy; miRNAs; miR-144; LncMIAT; isoproterenol

期刊名称：CELLS （ 影响因子：6.6； 五年影响因子：6.663 ）

ISSN：

年卷期： 2020 年 9 卷 5 期

页码：

收录情况： SCI

摘要： Cardiac hypertrophy is a common pathological condition and an independent risk factor that triggers cardiovascular morbidity. As an important epigenetic regulator, miRNA is widely involved in many biological processes. In this study, miRNAs expressed in rat hearts that underwent isoprenaline-induced cardiac hypertrophy were identified using high-throughput sequencing, and functional verification of typical miRNAs was performed using rat primary cardiomyocytes. A total of 623 miRNAs were identified, of which 33 were specifically expressed in cardiac hypertrophy rats. The enriched pathways of target genes of differentially expressed miRNAs included the FoxO signaling pathway, dopaminergic synapse, Wnt signaling pathway, MAPK (mitogen-activated protein kinase) signaling pathway, and Hippo signaling pathway. Subsequently, miR-144 was the most differentially expressed miRNA and was subsequently selected for in vitro validation. Inhibition of miR-144 expression in primary myocardial cells caused up-regulation of cardiac hypertrophy markers atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP). The dual luciferase reporter system showed that ANP may be a target gene of miR-144. Long non-coding RNA myocardial infarction associated transcript (LncMIAT) is closely related to heart disease, and here, we were the first to discover that LncMIAT may act as an miR-144 sponge in isoproterenol-induced cardiac hypertrophy. Taken together, these results enriched the understanding of miRNA in regulating cardiac hypertrophy and provided a reference for preventing and treating cardiac hypertrophy.

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