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Isolation, identification, biological estimation, and profiling of glucosinolates inIsatis indigoticaroots

文献类型： 外文期刊

作者： Guo, Qihui 1 ; Sun, Yanzhi 1 ; Tang, Qingjiu 2 ; Zhang, Hongchao 3 ; Cheng, Zhihong 1 ;

作者机构： 1.Fudan Univ, Sch Pharm, Dept Nat Med, 826 Zhangheng Rd, Shanghai 201203, Peoples R China

2.Shanghai Acad Agr Sci, Shanghai Edible Fungi Inst, Shanghai, Peoples R China

3.Shanghai Yeehope Biochem Co Ltd, Shanghai, Peoples R China

关键词： Isatis indigotica; glucosinolates; goitrin; QTOF-MS; MS; desulfurization

期刊名称：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES （ 影响因子：1.312； 五年影响因子：1.145 ）

ISSN： 1082-6076

年卷期： 2020 年 43 卷 15-16 期

页码：

收录情况： SCI

摘要： Four main glucosinolates (progoitrin, epiprogoitrin, gluconapin, and neoglucobrassicin) were isolated and identified fromIsatis indigoticaroots (Isatidis Radix). Among these, neoglucobrassicin was the first indole glucosinolate isolated from this herb, and its structure was elucidated by NMR, MS, and X-ray diffraction analysis. The glucosinolate components of Isatidis Radix have been profiled by combined use of a conventional preparative column chromatography method and a new UHPLC-QTOF-MS/MS method, and validated by simultaneous analysis of intact and desulfurized GLS. A total of 16 glucosinolates (GLS) including 12 aliphatic GLS, two aromatic GLS, and two indole GLS were identified on the basis of their UV profiles, MS/MS data, and/or by comparison with reference substances. Among these nine GLS were reported fromI. indigoticafor the first time. The total GLS, four isolated GLS, and (R,S)-goitrin (a degraded product of progoitrin and epiprogoitrin) have been estimatedin vitrofor anti-influenza viral, anti-bacterial, and anti-inflammatory activities. All the glucosinolate samples were inactive against H3N2 virus and four pathogenic bacteria, and two previously unnoticed GLS (gluconapin and neoglucobrassicin) showed significantly anti-inflammatory activity against the respiratory burst of mouse macrophages.

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