Physicochemical and Antioxidant Properties ofPotentilla anserinaL. Polysaccharides Affected by Ultrasonication
文献类型： 外文期刊
作者： Huang, Yulong 1 ; Guo, Jie 3 ; Zhang, Ji 3 ;
作者机构： 1.Northwest Normal Univ, Coll Chem & Chem Engn, Lanzhou 730070, Peoples R China
2.Gansu Acad Agr Sci, Agr Prod Storage & Proc Inst, Lanzhou 730070, Peoples R China
3.Northwest Normal Univ, Coll Life Sci, Bioact Prod Engn Res Ctr Gansu Distinct Plants, Lanzhou 730070, Peoples R China
关键词： physicochemical property; antioxidant activity; Potentilla anserinaL; polysaccharide; ultrasonic treatment
期刊名称：APPLIED SCIENCES-BASEL （ 影响因子：2.679； 五年影响因子：2.736 ）
ISSN：
年卷期： 2020 年 10 卷 13 期
页码：
收录情况： SCI
摘要： The effects of ultrasonic treatment on the physicochemical and antioxidant properties ofPotentilla anserinaL. polysaccharides (PAPs) were investigated. A Box-Behnken design (BBD) was applied to optimize the parameters of ultrasound-assisted extraction (UAE). A maximum yield of 9.43% was achieved at an extraction temperature of 66 degrees C, ultrasonic power of 205 W, and extraction time of 2.6 h. The chemical structure analyses illustrated that the two PAP extracts using UAE and hot water extraction (HWE) had the same monosaccharide composition but displayed a significant difference in the monosaccharide content, and the molecular weight of PAP(UAE)was significantly lower than PAP(HWE). Fourier-transform infrared (FT-IR) spectroscopy confirmed that the ultrasonic treatment did not change the type of glycosidic bonds compared with HWE. In addition, the results from thermogravimetric analysis indicated that different extraction methods had effects on the physical properties of PAPs to some degree. Antioxidant activity assays demonstrated that PAP(UAE)had higher superoxide dismutase and glutathione peroxidase activities when cells were exposed to exogenous H(2)O(2)than PAP(HWE), and the malondialdehyde levels were also lowered following exposure to PAP(UAE). These findings indicated that ultrasound irradiation is a promising technique for polysaccharide extraction from plant materials.
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