您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

De novo sequencing and chromosomal-scale genome assembly of leopard coral grouper,Plectropomus leopardus

文献类型： 外文期刊

作者： Zhou, Qian 1 ; Guo, Xinyu 3 ; Huang, Yang 5 ; Gao, Haoyang 3 ; Xu, Hao 1 ; Liu, Shanshan 3 ; Zheng, Weiwei 1 ; Zhang, Tia 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao, Peoples R China

2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China

3.BGI Shenzhen, BGI Qingdao, Qingdao, Peoples R China

4.BGI Shenzhen, Qingdao Europe Adv Inst Life Sci, Qingdao, Peoples R China

5.Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techn, Southern Marine Sci & Engn Guangdong Lab, Zhanjiang, Peoples R China

6.Sun Yat Sen Univ, Sch Life Sci, Guangzhou, Peoples R China

关键词： chromosomal assembly; genome annotation; genome sequencing; leopard coral grouper; Plectropomus leopardus

期刊名称：MOLECULAR ECOLOGY RESOURCES （ 影响因子：7.09； 五年影响因子：8.745 ）

ISSN： 1755-098X

年卷期： 2020 年 20 卷 5 期

页码：

收录情况： SCI

摘要： The leopard coral grouper,Plectropomus leopardus, belonging to the family Epinephelinae, is a carnivorous coral reef fish widely distributed in tropical and subtropical waters of the Indo-Pacific. Due to its appealing body appearance and delicious taste,P. leopardushas become a popular commercial fish for aquaculture in many countries. However, the lack of genomic and molecular resources forP. leopardushas hindered study of its biology and genomic breeding programmes. Here we report the de novo sequencing and assembly of theP. leopardusgenome using a combination of 10 x Genomics, high-throughput chromosome conformation capture (Hi-C) and PacBio long-read sequencing technologies. The genome assembly has a total length of 881.55 Mb with a scaffold N50 of 34.15 Mb, consisting of 24 pseudochromosome scaffolds.buscoanalysis showed that 97.2% of the conserved single-copy genes were retrieved, indicating the assembly was almost entire. We predicted 25,248 protein-coding genes, among which 96.5% were functionally annotated. Comparative genomic analyses revealed that gene family expansions inP. leoparduswere associated with immune-related pathways. In addition, we identified 5,178,453 single nucleotide polymorphisms based on genome resequencing of 54 individuals. TheP. leopardusgenome and genomic variation data provide valuable genomic resources for studies of its genetics, evolution and biology. In particular, it is expected to benefit the development of genomic breeding programmes in the farming industry.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台