Isolation and characterization of 100 SNP markers in lined seahorse (Hippocampus erectus) using RAD sequencing
文献类型： 外文期刊
作者： Zhu, Ke-Cheng 1 ; Ma, Qiwei 1 ; Zhang, Dian-Chang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, 231 Xingang Rd West, Guangzhou 510300, Peoples R China
2.Guangdong Prov Engineer Technol Res Ctr Marine Bi, Guangzhou 510300, Peoples R China
3.Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 511458, Peoples R China
4.Chinese Acad Fishery Sci, Trop Aquaculture Res & Dev Ctr, South China Sea Fisheries Res Inst, Sanya 572018, Peoples R China
关键词： Hippocampus erectus; SNP; RAD; Selective breeding; Conservation genetics
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）
ISSN： 1877-7252
年卷期： 2020 年 12 卷 4 期
页码：
收录情况： SCI
摘要： Lined seahorse (Hippocampus erectus), an important economic and medicinal fish in China, exists widely in Atlantic Ocean. However, habitat destruction for its spawning and overfishing has led to profound decline the wild populations in recent years. Therefore, to improve the molecular-assisted selection (MAS) and resources conservation ofH. erectus, the 100 novel single nucleotide polymorphism (SNP) loci are developed by restriction-site associated DNA (RAD) sequencing in this species. In the present study, the observed heterozygosity (Ho) and expected heterozygosity (H-E) varied from 0.0263 to 0.8154 and 0.0261 to 0.506, respectively. The minor allele frequency (MAF) ranged from 0.0132 to 0.5000. The PIC value was between with 0.0257 and 0.375. Fifteen loci displayed prominent deviations from the Hardy-Weinberg equilibrium (HWE) after Bonferroni correction (P < 0.05), but no remarkable linkage disequilibrium was observed. These novel isolated SNPs will be useful for selective breeding and conservation genetics studies of Seahorse.
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