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Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties

文献类型： 外文期刊

作者： Ma, Yan 1 ; Li, Jie 2 ; Zhang, Xin-Yue 1 ; Ni, Hao-Dong 1 ; Wang, Feng-Biao 1 ; Wang, Hai-Ying 2 ; Wang, Zhi-Peng 1 ;

作者机构： 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266109, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao 266071, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Sustainable Dev Polar Fishery, Qingdao 266071, Peoples R China

关键词： Alginate lyase; Intracellular; pH-stable; Metal ion-resisted; Yarrowia lipolytica

期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）

ISSN：

年卷期： 2020 年 18 卷 8 期

页码：

收录情况： SCI

摘要： Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 fromVibriosp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host,Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 degrees C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.

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