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Molecular characterization of GRP94 and HSP90 alpha from Trachinotus ovatus, Linnaeus 1758 and their expression responses to various levels of stocking density stress and Cryptocaryon irritans infection

文献类型： 外文期刊

作者： Yang, Quan 1 ; Zhu, Ke-Cheng 1 ; Guo, Liang 1 ; Liu, Bao-Suo 1 ; Guo, Hua-Yang 1 ; Zhang, Nan 1 ; Yang, Jing-Wen 1 ; Zhan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, Guangzhou 510300, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

3.Chinese Acad Fishery Sci, Trop Aquaculture Res & Dev Ctr, South China Sea Fisheries Res Inst, Sanya 572018, Peoples R China

4.Guangdong Prov Engineer Technol Res Ctr Marine Bi, Guangzhou, Guangdong, Peoples R China

5.Sanya Trop Fisheries Res Inst, Sanya, Hainan, Peoples R China

关键词： Trachinotus ovatus; GRP94; HSP90 alpha; Stocking density; Cryptocaryon irritans

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2020 年 529 卷

页码：

收录情况： SCI

摘要： Heat shock proteins (HSPs) are a class of molecular chaperones with a wide range of biological activities. To understand the stress responses of 94-kDa glucose-regulated protein (GRP94) and heat shock protein 90 alpha (HSP90 alpha) under stocking density stress and C. irritans infection, we obtained their cDNA sequences from T. ovatus and performed bioinformatic analysis on their structures. Meanwhile, the tissue-specific expression patterns of these genes were also detected by qPCR. The ToGRP94 cDNA contains an open reading frame of 2394 bp, which encodes a protein of 797 amino acids, while ToHSP90 alpha contains an open reading frame of 2190 bp, which encodes a protein of 730 amino acids. Amino acid sequence analysis showed that these proteins have highly conserved characteristic family domains or motifs, such as the heat shock hsp90 protein family signature (Y [k/s] NKEIFLRE). ToGRP94 was recently related to S. dumerili GRP94 with amino acid sequence homology up to 96.86%, and ToHSP90 alpha had a higher evolutionary correlation with M. armatus and A. testudineus HSP90 alpha with sequence homology of 98.49% and 97.67%, respectively. ToGRP94 and ToHSP90 alpha mRNAs were widely expressed in all tissues tested under normal conditions. ToGRP94 mRNA was most strongly expressed in gill tissue followed by fin and kidney tissues, while ToHSP90 alpha was most strongly expressed in blood tissues followed by brain, kidney and gill tissues. Under stocking density stress, ToGRP94 mRNA in the liver was significantly higher in the LD group, but it did not change in the kidney and spleen tissues with increasing stocking density; however, the expression level of ToHSP90 alpha gene increased significantly in liver, spleen, and kidney tissues under high stocking densities, which indicates that ToHSP90 alpha was closely related to density chronic stress. After C. irritans infection, the expression of ToGRP94 was significantly increased in liver, spleen, and kidney tissues. Similarly, ToHSP90 alpha gene expression was significantly increased in liver and kidney tissues, but there was no difference in spleen tissue. The results indicate that these two genes may be involved in the immune response of T. ovatus. In summary, ToHSP90 alpha would be involved in chronic stress and the immune response, while ToGRP94 would be mainly involved in the immune regulatory response. These results may provide a reference for further research studying the function of fish HSP90 and its mechanism under environmental stress.

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