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Characterization of novel antigenic vaccine candidates for nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection

文献类型： 外文期刊

作者： Ma, Yanping 1 ; Hao, Le 1 ; Liang, Zhiling 1 ; Ma, Jiangyao 1 ; Ke, Hao 1 ; Kang, Huahua 1 ; Yang, Hongwei 5 ; Wu, Jing 6 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China

2.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou, Peoples R China

3.Minist Agr & Rural Affairs, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou, Peoples R China

4.Guangdong Lab Lingnan Modern Agr, Zhaoqing Branch, Zhaoqing, Peoples R China

5.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou, Peoples R China

6.South China Agr Univ, Coll Vet Med, Guangzhou, Peoples R China

关键词： Streptococcus agalactiae; Candidate subunit vaccine; Immunogenic proteins; Immune responses; Protection

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2020 年 105 卷

页码：

收录情况： SCI

摘要： Streptococcus agalactiae is one of the important pathogens responsible for high mortality and economic losses of the tilapia industry worldwide. Based on ten serovars of S. agalactiae infection, subunit vaccine with conserved antigens is promising strategy corresponding stimulated long-term immunity and provides protection for animals against different serotypes of S. agalactiae. In the present study, eight proteins (AP, AL, LivK, ESAT6, essA, essB, essC and esaA) were selected from the S. agalactiae serotype Ia genome as immunogenic antigens with bioinformation and immune experiment assays. These recombinant proteins were successfully obtained through expression in Escherichia coli and the immunogenicity was assessed in tilapia challenge model. The results showed that the recombinant proteins caused high-level-specific antibodies production and high lysozyme activities, suggesting that the recombinant proteins induced specific humoral immune response and innate immune response of tilapia. The signficant increase were observed in the cytokines levels of TNF-alpha, IL-1 beta, IFN-gamma, cc1, cc2 and immune-related genes levels of CD8 alpha and MHC factors in the spleen and head kidney tissues, suggesting that the recombinant proteins induced immune response of tilapia through cytokines signal pathway and activated high cytotoxic T-lymphocyte (CTL) activity of tilapia. Furthermore, vaccinated tilapia conferred high levels of protection against challenge with a lethal dose of highly virulent serovar Ia (highest RPS was 91.60% in AL and essC protein groups). Our results indicated that the eight recombinant proteins induced high level of immune responses and offered protection against S. agalactiae infection, could be potential subunit vaccine candidates.

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