Deep illumina miRNA sequencing provides insights into the mechanism underlying grass carp reovirus infection
文献类型： 外文期刊
作者： Ge, Ye 1 ; Yao, Qiucheng 1 ; Wang, Yingying 2 ; Wang, Qing 2 ; Zeng, Weiwei 2 ;
作者机构： 1.Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol, Key Lab Fishery Drug Dev,Minist Agr, Guangzhou 510380, Guangdong, Peoples R China
3.Foshan Univ, Sch Life Sci & Engn, Foshan, Peoples R China
4.Foshan Univ, Guangdong Prov Key Lab Anim Mol Design & Precise, Foshan, Peoples R China
关键词： GCRV; microarray sequencing; miRNA; qPCR
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）
ISSN： 1355-557X
年卷期：
页码：
收录情况： SCI
摘要： GCRV virulent strain HuNan1307 was infected with healthy grass carp. After 3 weeks, small RNA from kidney tissue of the infected group and the control group was extracted and microarray sequencing was conducted to complete the construction of miRNA libraries of various organs of GCRV virulent strain HuNan1307 and functional annotation of miRNA in the expression profile. Through differential comparison, analysis and verification, miRNAs with many differences between the infection group and the control group were screened out. We found 2,717 miRNAs were detected in the five groups and 14,460 miRNA target genes were predicted and 29 co-expressions of miRNAs in grass carp infected GCRV with RNA-Seq. Integrated miRNA expression profiling and pathway analysis show immune effect process, regulation of immune effect process, cell death and regulation of leucocyte-mediated cytotoxicity, and the PI3K/AKT, Toll-like and RIG-I pathways were potentially deregulated. Real-time quantitative PCR was performed on selected miRNAs in order to validate their expression. Six miRNAs were chosen to verify the result of the deep illumina miRNA sequencing using real-time PCR method. The result showed that miR710 and miR2696 had the coincide result with deep illumina miRNA sequencing. The development of this study is the basis for understanding the replication, infection, immunity, pathogenesis and transmission of GCRV. This result will help to understand the gene regulation of miRNA between GCRV infection and the host, which contribute to develop new anti-GCRV drugs.
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