文献类型： 外文期刊
作者： Wu, Wenmei 1 ; Li, Kang 3 ; Zhao, Haigang 3 ; Xu, Xianying 1 ; Xu, Jing 1 ; Luo, Man 1 ; Xiao, Yang 4 ; Tian, Ling 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Sericulture & Mulberry Engn Res Ct, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Peoples R China
2.Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangdong Prov Key Lab Biotechnol Drug Candidates, Guangzhou 510006, Peoples R China
3.South China Normal Univ, Sch Life Sci, Inst Insect Sci & Technol, Guangdong Prov Key Lab Insect Dev Biol & Appl Tec, Guangzhou 510631, Peoples R China
4.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Peoples R China
关键词： BmTip60; autophagy; Bombyx mori; AMP-activated protein kinase; starvation
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2020 年 21 卷 18 期
页码：
收录情况： SCI
摘要： Tip60, a key histone acetyltransferase of the MYST family and member of the nuclear multimeric protein complex (NuA4), regulates the activity and stability of proteins involved in the cell cycle, DNA damage responses, autophagy, etc. However, the function and regulatory mechanism of Tip60 homolog in Bombyx mori are not elucidated. In the present study, Bombyx Tip60 (BmTip60) was functionally identified. Developmental profiles showed that the protein levels and nuclear localization of BmTip60 peaked in fat body during the larval-pupal metamorphosis when autophagy was intensive; simultaneously, the BmTip60 protein migrated to form an upper band as detected by Western blot. Interestingly, the upper band of BmTip60 was reduced by lambda-phosphatase treatment, indicating that it was a phosphorylated form of BmTip60. Results showed that BmTip60 was promoted by starvation but not 20-hydroxyecdysone treatment. Transcription factor AMP-activated protein kinase (AMPK) affected by starvation was pivotal for BmTip60 protein migration. In addition, one mammalian phosphorylation site was identified in BmTip60 at Ser99, the constitutive-activation mutation of Ser99 to Asp99 but not its inactive mutation to Ala99 significantly upregulated autophagy, showing the critical role of phosphorylation at Ser99 for BmTip60-mediated autophagy. In conclusion, the starvation-AMPK axis promotes BmTip60 in B. mori, which was requisite for autophagy induction. These results reveal a regulatory mechanism of histone acetyltransferase Tip60 homologs by phosphorylation in insects, and sheds light on further related studies of acetylation regulation.
- 相关文献
作者其他论文 更多>>
-
Mulberry leaf extract reduces damage by regulating the oxidative response, immune response and intestinal flora of largemouth bass (Micropterus salmoides) under chronic heat stress
作者：Fu, Bing;Zhou, Donglai;Qiu, Xiaotong;Zheng, Jieqiu;Yang, Qiong;Xiao, Yang;Liao, Sentai;Li, Qingrong;Xing, Dongxu;Qiu, Xiaotong;Zheng, Jieqiu
关键词：mulberry leaf extract; largemouth bass; heat stress; intestinal flora; liver injury; immune ability
-
Baculovirus protein kinase 1 activates AMPK-protein phosphatase 5 axis to hijack transcription factor EB for self-proliferation
作者：Pan, Jiasheng;Li, Rongsong;Chen, Yin;Memon, Fareed Uddin;Wu, Kun;Hu, Jianghao;Xie, Xiaole;Deng, Jianhao;Xu, Rongting;Tian, Ling;Pan, Jiasheng;Li, Rongsong;Chen, Yin;Memon, Fareed Uddin;Wu, Kun;Hu, Jianghao;Xie, Xiaole;Deng, Jianhao;Xu, Rongting;Tian, Ling;Li, Xiaodong;Li, Kang;Li, Kang;Li, Kang;Zhang, Chuanxi
关键词：BmNPV protein kinase 1; B. mori serine/threonine protein phosphatase 5; BmTFEB; Dephosphorylation; Proliferation
-
ANAPHASE-PROMOTING COMPLEX/CYCLOSOME coactivators maintain AURORA 1 kinase homeostasis during meiotic chromosome segregation
作者：Xu, Jing;Chen, Kaixin;Huang, Runsen;Wang, Yingxiang;Xu, Jing;Zhou, Lian;Niu, Baixiao;Ye, Juanying;Ma, Hong;Copenhaver, Gregory P.;Copenhaver, Gregory P.;Copenhaver, Gregory P.
关键词：
-
Lipid metabolites affected by deficient autophagy antagonize the occurrence of autophagy through AMPK signaling in insects
作者：Tian, Ling;Zhong, Qien;Yang, Yubei;Tian, Ling;Zhong, Qien;Yang, Yubei;Tian, Ling;Zhong, Qien;Yang, Yubei;Wu, Wenmei;Xiao, Yang;Li, Sheng;Li, Kang;Li, Kang
关键词：Autophagy;
Bombyx mori ;Drosophila melanogaster ; Lipid degradation; Metabolites -
Efficiency of microbial fermentation on microbial shifts, enzymatic activity, and transcriptions in black soldier fly larvae during the sugarcane waste conversion
作者：Memon, Fareed Uddin;Ahmad, Sheraz;Cui, Ying;Feng, Xingbao;Zeng, Peng;Huang, Zhijun;Tian, Ling;Memon, Fareed Uddin;Ahmad, Sheraz;Cui, Ying;Feng, Xingbao;Zeng, Peng;Huang, Zhijun;Tian, Ling;Li, Chiho;Nabi, Farhan;Tettamanti, Gianluca;Tettamanti, Gianluca;Chen, Ting
关键词：Microbial fermentation; Sugarcane waste; Black soldier fly; Microbial shifts; Lignocellulose degradation; Transcriptional profiles
-
Transcriptomics and Metabolomics Combined to Analyze the Response Mechanism of Silkworm Eggs to High-Temperature Stress
作者：Xiao, Yang;Li, Qingrong;Sun, Zhenbo;Fu, Bing;Yang, Qiong;Zhang, Weilong;Xing, Dongxu;Jiang, Mangui;Huang, Xuhua
关键词：transcriptomics; metabonomics; silkworm egg; instant acid-impregnated eggs; high temperature stress
-
Hyperthermia-induced transcriptome remodeling impairs embryo viability in Bombyx mori
作者：Sun, Zhenbo;Yang, Qiong;Xiao, Yang;Li, Qingrong;Zhang, Weilong;Xing, Dongxu;Li, Ying;Liu, Xinlei;Duan, Jianping
关键词：Bombyx mori; Egg diapause; Thermal stress; Full-length transcriptome; Heat shock protein
