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Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on Lentinula edodes and Pleurotus eryngii Strains

文献类型： 外文期刊

作者： Chen, Zhanghu 1 ; Yang, Ruiheng 1 ; Li, Yan 1 ; Tang, Lihua 1 ; Xiong, Huiyang 1 ; Bao, Dapeng 1 ; Guo, Ting 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Minist Agr, Natl Res Ctr Edible Fungi Biotechnol & Engn, Key Lab Appl Mycol Resources & Utilizat,Shanghai K, Shanghai 201403, Peoples R China

2.Shanghai Ocean Univ, Coll Food Sci, Shanghai 201306, Peoples R China

3.Zhaotong Univ, Yunnan Key Lab Gastrodia & Fungi Symbiot Biol, Zhaotong 657000, Peoples R China

关键词： Lentinula edodes; Pleurotus eryngii; sclerotium; transcriptome; medium composition; energy metabolism

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 10 卷 12 期

页码：

收录情况： SCI

摘要： In the industrial production of Lentinula edodes and Pleurotus eryngii, slow growth of the mother seed and insufficient hyphal vitality can significantly affect the cultivation process. To shorten the growth period on traditional PDA medium, two strains of L. edodes and P. eryngii were cultured with different proportions of P. tuber-regium and Wolfiporia hoelen sclerotium powders added into the medium to investigate the effect on the mycelial growth. Compared to the PDA, the addition of sclerotia powder significantly enhanced the growth of mycelia, with an optimal addition ratio of 2%. Transcriptome sequencing was performed after culturing L. edodes and P. eryngii on PDA, PDA with 2% P. tuber-regium sclerotium powder, and PDA with 2% W. hoelen sclerotium powder. GO enrichment analysis of the differentially expressed genes (DEGs) of L. edodes and P. eryngii strains cultured in the sclerotia powder media showed significant changes in oxidoreductase and glucosidase activities. Changes were observed in KEGG annotation for carbohydrate metabolism, glycolysis, pyruvate metabolism, and other energy metabolic pathways. Moreover, carbohydrate-active enzyme (CAZyme) family genes were predominantly upregulated. The increase in the activity of CAZyme and oxidoreductases promotes the degradation of nutrients in the sclerotia into small-molecule substances, which explains why the sclerotia powder culture medium promotes mycelial growth.

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