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Identification and functional characterization of two Bcl-2 family proteins in swimming crab, Portunus trituberculatus

文献类型： 外文期刊

作者： Zhang, Yunbin 1 ; Wang, Lei 1 ; Xu, Yao 1 ; Ren, Xianyun 1 ; Liu, Ping 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China

3.Shanghai Ocean Univ, Natl Expt Teaching Demonstrat Ctr Aquat Sci, Shanghai, Peoples R China

4.Jiangsu Ocean Univ, Bioresources & Environm, Jiangsu Key Lab Marine Biotechnol, Lianyungang, Peoples R China

关键词： Portunus trituberculatus; Bcl-2 family proteins; Gene silencing; Apoptosis; Immune defenses; Protein interactions

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2022 年 553 卷

页码：

收录情况： SCI

摘要： Bcl-2 family proteins are regulatory factors of the mitochondrial apoptosis pathway that are vital to immune defenses and many other biological processes. However, the effects of Bcl-2 family proteins on apoptosis and immunity in crustaceans is poorly studied. In the study, two novel genes including Bax gene (PtBax) and Bcl-2 (PtBcl-2) from Portunus trituberculatus were identified and characterized. The deduced amino acid sequences showed that PtBax and PtBcl-2 possess domains of typical Bcl-2-related proteins. Both genes were widely expressed in all tested tissues, with highest expression levels in the hepatopancreas. Fluorescence in situ hybridization revealed that PtBax and PtBcl-2 were mainly expressed in the cytoplasm, where they play an important role in pathogen infection. Both PtBax and PtBcl-2 were significantly upregulated in the hepatopancreas at both mRNA and protein levels following challenge with Vibrio parahaemolyticus or white spot syndrome virus (WSSV). Moreover, double-stranded RNA-mediated silencing of both genes altered apoptosis levels in the hepatopancreas and WSSV copy number in muscles. Interactions between PtBax and PtBcl-2 were analyzed at transcriptional and protein levels. Yeast two-hybrid system analysis showed that PtBcl-2 could interact with PtBax directly. These results suggest that PtBax and PtBcl-2 may participate in pathogen infection by regulating apoptosis.

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