文献类型： 外文期刊
作者： Huang, Xuan-yun 1 ; Huang, Dong-mei 1 ; Shi, Yong-fu 1 ; Feng, Bing 1 ; Yu, Hui-juan 1 ; Cai, You-qiong 1 ;
作者机构： 1.Minist Agr PR China, CAFS Aquat Prod Qual Inspect & Test Ctr Shanghai, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
关键词： Ciprofloxacin;Hapten;Conjuguate;Antibody
期刊名称：ADVANCES IN CHEMICAL ENGINEERING III, PTS 1-4
ISSN： 1022-6680
年卷期： 2013 年 781-784 卷
页码：
收录情况： SCI
摘要： In order to generate the specific antibody of small molecular hapten-ciprofloxacin for detecting the Ciprofloxacin residues in edible animal tissues. A modified Carbodiimide coupling method was developed to obtain the immunogenic conjugates through coupling ciprofloxacin with bovine serum albumin in five different proportions. The conjugates were characterized and verified by electrophoresis, the trinitro-benzene-sulfonic acid (TNBS) method and ultraviolet spectra scanning. A quantitative analysis of the density of ciprofloxacin-bovine serum albumin conjugates suggested that a high conjugate density 30:1 could be obtained in the optimized conditions. By immunizing the mice, the antibody showed high sensitivity toward ciprofloxacin with an IC50 of 4.69 ng/ml. This study confirmed a practical approach for the ciprofloxacin-bovine serum albumin conjugates, through which the high potent antisera specific for ciprofloxacin hapten can be produced for immunoassay.
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