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High-throughput sequencing of 16S rRNA gene analysis reveals novel taxonomic diversity among vaginal microbiota in healthy and affected sows with endometritis

文献类型： 外文期刊

作者： Liang, Huixian 1 ; Cai, Rujian 2 ; Li, Chunling 2 ; Glendon, Ong Hong Ming 5 ; Hu Chengcheng 5 ; Yan, He 1 ;

作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Guangdong, Peoples R China

3.Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou, Peoples R China

4.Guangdong Open Lab Vet Publ Hlth, Guangzhou, Peoples R China

5.Sino Singapore Int Joint Res Inst SSIJRI, Guangzhou 510000, Peoples R China

6.Nanyang Technol Univ, Sch Chem & Biomed Engn, Block N1-2,B3-15,62 Nanyang Dr, Singapore 637459, Singapore

7.Singapore Inst Mfg Technol, 2 Fusionopolis Way,08-04 Innovis, Singapore 138634, Singapore

关键词： Endometritis; Sows; 16S rRNA sequencing; Vaginal microbiota; Burkholderia

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.554； 五年影响因子：2.564 ）

ISSN： 0034-5288

年卷期： 2022 年 143 卷

页码：

收录情况： SCI

摘要： In sows afflicted with endometritis, vaginal microbiota can provide valuable information regarding bacterial community diversity. Our aim was to compare the vaginal microbiotas between endometritis and healthy sows and characterize the vaginal microbiota of endometritis sows using high-throughput sequencing of the 16S rRNA gene. Vaginal swabs were collected from healthy (Healthy_A, n = 10; Healthy_B, n = 10) and diseased (Endometritis_A, n = 10; Endometritis_B, n = 10) sows from two swine farms located in Guangdong and Yunnan province, in Southern China. The results of V3-V4 region of the 16S rRNA gene showed that Corynebacterium_1, Clostridium_sensu_stricto_1, Porphyromonas, Anaerococcus, Streptococcus, and Bacteroides comprised the core microbiota in all healthy sows. Two type of endometritis microbiota patterns were presented in two farms: the first comprised mostly of Burkholderia in farm A and the second comprised of Parvimonas in farm B. In farm A, the percentages of Burkholderia, Serratia, and Enterobacter were higher in the endometritis group, while only Parvimonas was significantly increased in the endometritis group in farm B (p < 0.05). Interestingly, the genus Burkholderia and Serratia were found only in the endometritis sows from farm A. Burkholderia was the most dominant genus found in endometritis sows and was confirmed by full-length 16S rRNA analysis using PacBio sequencing.

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