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Protective immunity against iridovirus disease in mandarin fish, induced by recombinant major capsid protein of infectious spleen and kidney necrosis virus

文献类型： 外文期刊

作者： Fu, Xiaozhe 1 ; Li, Ningqiu 1 ; Lai, Yingtiao 1 ; Liu, Lihui 1 ; Lin, Qiang 1 ; Shi, Cunbin 1 ; Huang, Zhibin 1 ; Wu, Shu 1 ;

作者机构： 1.CAFS, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Drug Dev, MOA, Guangzhou 510380, Guangdong, Peoples R China

关键词： Mandarin fish;Infectious spleen and kidney necrosis virus;Major capsid protein;Humoral immunity;Cellar immunity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2012 年 33 卷 4 期

页码：

收录情况： SCI

摘要： Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a disease causing high mortality and economic losses in mandarin fish, Siniperca chuatsi in China. But little information about vaccine development against ISKNV disease is available. In this study the gene encoding the major capsid protein (MCP), which is predominant structural component of the iridovirus particles, was cloned into a temperature induction prokaryotic expression vector pBV220 and a recombinant protein was detected about 50 kDa in molecular weight and accounted for 23% of total proteins of whole cell. Polyclonal antibodies were raised in rabbits against the purified protein and the reaction of the antibody was confirmed by western blotting using the purified protein and the spleen and kidney of healthy and diseased mandarin fish. The recombinant protein was renatured by dialysis and the juvenile mandarin fish were vaccinated by intraperitoneal injection with recombinant MCP emulsified with ISA 763 adjuvant at a dose of 20 mu g/fish, 50 mu g/fish and 100 mu g/fish, respectively. Specific antibodies and lymphocyte proliferation were detected in three groups and the values of MCP50 group were higher than the other two groups. After challenge infection with ISKNV, fish of MCP50 group showed significantly greater survival than the others and the RPS was 64.3%. In conclusion, the humoral immunity and cellar immunity of mandarin fish were induced by recombinant MCP and the best immune dose was 50 mu g/fish. To the best of our knowledge, this is the first time that a recombinant protein vaccine against ISKNV disease was developed in mandarin fish. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Protective immunity against infectious spleen and kidney necrosis virus induced by immunization with DNA plasmid containing mcp gene in Chinese perch Siniperca chuatsi. Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Guo, Huizhi,Zhang, Defeng,Liu, Lihui,Wu, Shuqin,Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Zhang, Defeng,Liu, Lihui,Wu, Shuqin.

[2]Vaccination with Recombinant Baculovirus Expressing Ranavirus Major Capsid Protein Induces Protective Immunity in Chinese Giant Salamander, Andrias davidianus. Zhou, Xiaoyuan,Zhang, Xinglang,Han, Yahui,Jia, Qiuhong,Gao, Hongwei. 2017

[3]Protein encoded by ORF093 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in Chinese perch Siniperca chuatsi. Li, Ningqiu,Fu, Xiaozhe,Guo, Huizhi,Lin, Qiang,Liu, Lihui,Zhang, Defeng,Fang, Xiang,Wu, Shuqin,Li, Ningqiu,Fu, Xiaozhe,Guo, Huizhi,Lin, Qiang,Liu, Lihui,Zhang, Defeng,Fang, Xiang,Wu, Shuqin.

[4]Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China. Huang, X.,Huang, Y.,Wei, S.,Ouyang, Z.,Qin, Q.,Xu, L.,Feng, J..

[5]Early protein ORF086 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in mandarin fish Siniperca chuatsi. Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Guo, Huizhi,Liu, Lihui,Huang, Zhibin,Wu, Shuqin,Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Guo, Huizhi,Liu, Lihui,Wu, Shuqin.

[6]Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection. Tu, Jiagang,Chen, Wenjie,Zhao, Lijuan,Lan, Jiangfeng,Lin, Li,Fu, Xiaozhe,Lin, Qiang,Chang, Ouqin,Li, Ningqiu. 2016

[7]Genotype and host range analysis of infectious spleen and kidney necrosis virus (ISKNV). Fu, Xiaozhe,Li, Ningqiu,Liu, Lihui,Lin, Qiang,Wang, Fang,Lai, Yingtiao,Jiang, Haiming,Pan, Houjun,Shi, Cunbin,Wu, Shuqin. 2011

[8]Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation. Tian, J. Y.,Xie, H. X.,Zhang, Y. A.,Xu, Z.,Yao, W. J.,Nie, P.,Tian, J. Y.,Xie, H. X.,Zhang, Y. A.,Xu, Z.,Yao, W. J.,Nie, P.,Tian, J. Y..

[9]Isolation, pathogenicity and characterization of a novel bacterial pathogen Streptococcus uberis from diseased mandarin fish Siniperca chuatsi. Luo, Xia,Fu, Xiaozhe,Liao, Guoli,Chang, Ouqin,Huang, Zhibin,Li, Ningqiu,Fu, Xiaozhe,Li, Ningqiu.

[10]Investigating the potential immune role of fish NCAMs: Molecular cloning and expression analysis in mandarin fish. Wu, Nan,Zhang, Xiang-Yang,Zhang, Nu,Zhang, Xu-Jie,Guo, Xia,Chen, Xiao-Ling,Zhang, Yu,Wu, Han,Li, Shun,Li, Ai-Hua,Zhang, Yong-An,Zhang, Xiang-Yang,Zhang, Nu,Huang, Bei,Zhang, Xu-Jie,Guo, Xia.

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