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Immune and regulative characterization of complement-related gene cfhl5 in response to Vibrio harveyi challenge in Cynoglossus semilaevis

文献类型： 外文期刊

作者： Ma, Xinran 1 ; Wei, Min 1 ; Chen, Huijuan 1 ; Zhang, Junwei 2 ; Chen, Quanchao 2 ; Chen, Songlin 2 ; Zhou, Qian 2 ;

作者机构： 1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Biol Resources & Environm, Lianyungang 222000, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Shandong, Peoples R China

3.Qingdao Marine Sci & Technol Ctr, China Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China

关键词： Cynoglossus semilaevis; cfhl5; Regulative characterization; Immune response; DNA methylation

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.1； 五年影响因子：4.2 ）

ISSN： 1050-4648

年卷期： 2024 年 152 卷

页码：

收录情况： SCI

摘要： Complement factor H-related protein (CFHR) plays an important role in regulating complement activation and defensive responses. The function of CFHR2 (complement factor H related 2), a member of the CFHR family, in fish remains unclear. Here, we report the genetic relationship, expression characteristics and regulatory mechanism of cfhl5 (complement factor H like 5) gene, which encodes CFHR2 in Chinese tongue sole. We observed that the cfhl5 gene was widely expressed in several tissues, such as brain, heart and immune organs, and was most abundantly expressed in liver. After injection with Vibrio harveyi, the expression of cfhl5 was up-regulated significantly in liver, spleen and kidney at 12 or 24 hours post infection (hpi), suggesting an involvement of this gene in the acute immune response. Knockdown of cfhl5 in liver cells significantly up-regulated the expression of the pro-inflammatory cytokines tnf-alpha (tumor necrosis factor-alpha) and il1 beta (interleukin-1beta), the immunomodulatory factor il10 (interleukin-10) and the lectin complement pathway gene masp1 (MBL-associated serine protease 1), and down-regulated the expression of complement components c3 (complement 3) and cfi (complement factor I). In our previous work, we found that cfhl5 gene was significantly higher methylated and lower expressed in the resistant family compared with the susceptible family. Therefore, we used dual-luciferase reporter system to determine the effect of DNA methylation on this gene and found that DNA methylation could inhibit the promoter activity to reduce its expression. These results demonstrated that the expression of cfhl5 is regulated by DNA methylation, and this gene might play an important role in the immune response by regulating the expression of cytokines and complement components genes in Chinese tongue sole.

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