文献类型： 外文期刊

作者： Ji, Zhehui ¹ ; Zhu, Chuanzhong ² ; Zhu, Xinyang ² ; Ban, Sainan ³ ; Yu, Lijuan ¹ ; Tian, Juan ¹ ; Dong, Lixue ¹ ; Wen, Hua ¹ ; Lu, Xing ¹ ; Jiang, Ming ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China

2.Fujian DBN HY Aquat Sci & Technol Grp Co Ltd, Fujian Key Lab Funct Aquafeed & Culture Environm C, Zhaoan, Peoples R China

3.Beijing DBN Technol Grp Co Ltd, Beijing, Peoples R China

关键词： Bacillus subtilis; Intestinal microbiota; Intestinal health; Disease resistance; Chinese perch(Sinipercachuatsi)

期刊名称：ANIMAL NUTRITION （ 影响因子：6.3； 五年影响因子：6.9 ）

ISSN： 2405-6383

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： The purpose of this study was to evaluate the potential of a host-associated Bacillus subtilis 1-C-7 as a probiotic for Chinese perch (Siniperca chuatsi). Four test diets were formulated to contain graded levels of B. subtilis 1-C-7 at 0 (CY), 0.85 x 108 (Y1), 0.95 x 109 (Y2) and 0.91 x 1010 (Y3) CFU/kg diet. The test fish with initial weight 30.0 +/- 1.2 g were fed the 4 test diets with 3 replicates in an indoor water-flow aquaculture system with 12 net cages (40 fish/cage) for 10 wk. At the conclusion of the feeding trial, the probiotic effects of B. subtilis on Chinese perch were analyzed based on growth performance, serum biochemical indices, histologic morphology of liver and gut, gut microbiota and the resistance to Aero-monas hydrophila. The results showed that the percentage of weight gain had no significant change in the Y1 and Y2 groups (P > 0.05) but decreased in the Y3 group compared to that in the CY group (P < 0.05). The fish in the Y3 group displayed the highest activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) among these 4 groups (P < 0.05). The fish in the CY group had the highest value of malondialdehyde in the liver (P < 0.05) and showed severe nuclear migration and vacuolization of hepatocytes. The morphology indicated that all test fish had poor intestinal health. However, the fish in the Y1 group had a relatively normal intestinal histologic structure. The mid gut microbial diversity analysis showed that dietary B. subtilis supplementation increased the abundance of probiotics such as Tenericutes and Bacteroides, whereas it reduced the abundance of pernicious bacteria such as Proteobacteria, Actinobacteria, Thermophilia and Spirochaetes. The challenge test showed that dietary B. subtilis supplementation increased the resistance to A. hydrophila in Chinese perch. In conclusion, dietary supplementation of 0.85 x 108 CFU/kg B. subtilis 1-C-7 could improve the intestinal microbiota, intestinal health and disease resistance in Chinese perch, but more or excessive supple-mentation could reduce growth performance and have negative effects on health.(c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).