Production and verification of heterozygous clones in Japanese flounder, Paralichthys olivaceus by microsatellite marker
文献类型: 外文期刊
作者: Liu, Yongxin 2 ; Wang, Guixing 3 ; Liu, Yi 4 ; Hou, Jilun 4 ; Wang, Yufen 3 ; Si, Fei 3 ; Sun, Zhaohui 3 ; Zhang, Xiaoyan 3 ;
作者机构: 1.Chinese Acad Fishery Sci, Res Ctr Fisheries Recourse & Environm, Beijing 100141, Peoples R China
2.Chinese Acad Fishery Sci, Biotechnol Res Ctr, Beijing 100141, Peoples R China
3.Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
4.NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China
关键词: Japanese flounder;microsatellite;clone;gynogenesis
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2011 年 10 卷 75 期
页码:
收录情况: SCI
摘要: Mitotic gynogenetic diploids Japanese flounder Paralichthys olivaceus were produced by activating eggs with ultra violet (UV) irradiated sperm of red sea bream (Pagrus major), followed by hydrostatic pressure treatment to block the first mitotic division. By crossing two mitotic gynogenetic diploid females with two males, two heterozygous clones of Japanese flounder were produced. Microsatellite marker was used to confirm the genetic status of maternal parents and their progenies. 20 polymorphic microsatellite markers were chosen, covering 16 out of the total 24 linkage groups. The four maternal parents used in this experiment were completely heterozygous, while four mitotic gynogenetic diploids were homozygous for each marker. The genotypes of heterozygous clone progenies were identical and the combination of parental alleles, demonstrates the successful development of cloning.
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