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Production and confirmation of clones using gynogenesis in Japanese flounder

文献类型: 外文期刊

作者: Liu, Yongxin 2 ; Wang, Guixing 3 ; Liu, Yi 4 ; Hou, Jilun 4 ; Wang, Yufen 3 ; Si, Fei 3 ; Sun, Zhaohui 3 ; Zhang, Xiaoyan 3 ;

作者机构: 1.Chinese Acad Fishery Sci, Res Ctr Fisheries Recourse & Environm, Beijing 100141, Peoples R China

2.Chinese Acad Fishery Sci, Biotechnol Res Ctr, Beijing 100141, Peoples R China

3.Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China

4.NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China

关键词: Japanese flounder;microsatellite;clone;gynogenesis

期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )

ISSN: 1684-5315

年卷期: 2011 年 10 卷 57 期

页码:

收录情况: SCI

摘要: Two homozygous clones of Japanese flounder were produced by the gynogenesis that suppresses the cleavage of the eggs in the mitosis and then blocks the extrusion of the second polar body in the meiosis. Microsatellite was used to identify the genetic status of maternal parents and their progenies. 21 polymorphic microsatellite markers were chosen, distributed at the distal region of the 16 linkage group. In this experiment, the two used maternal parents were completely heterozygous, while both mitotic gynogenetic diploids and their clone progenies were homozygous for each marker, demonstrating the successful development of cloning.

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