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Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application

文献类型： 外文期刊

作者： Ding, Fuhong 1 ; Lall, Santosh P. 2 ; Li, Jun 3 ; Lei, Jilin 1 ; Rommens, Melissa 4 ; Milley, Joyce E. 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellowsea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 3Z1, Canada

3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

4.Scotian Halibut Ltd, Clarks Harbour, NS B0W 1P0, Canada

关键词： Atlantic halibut;Hippoglossus hippoglossus, L.;Cryopreservation;Sperm;Fertilization;Motility

期刊名称：CRYOBIOLOGY （ 影响因子：2.487； 五年影响因子：2.641 ）

ISSN： 0011-2240

年卷期： 2011 年 63 卷 1 期

页码：

收录情况： SCI

摘要： Development of Atlantic halibut (Hippoglossus hippoglossus) aquaculture will be enhanced with cryopreservation of halibut sperm by ensuring a reliable supply of sperm of desired quality and quantity. To assist in its commercial application, the cryopreservation of large volumes of halibut sperm was investigated. Three cryoprotectants were compared: dimethyl sulfoxide (DMSO), polyethylene glycol (PG) and glycerol (GLY) at two concentrations (10% or 15%). Two salt solutions, Hanks' balanced salt solution (HBSS) and 0.1 M KHCO(3) with 0.125 M sucrose solution (KS) were tested as diluents. Both factors were examined in 1.6 mL volumes. A cryopreservation volume of 4 mL and a low dilution ratio (1:1) were examined separately. Based on motility and fertilization rate, 10% and 15% DMSO diluted with HBSS or KS solution proved to be effective extenders with mean fertilization rates ranging from 52.2 +/- 27.2% to 65.8 +/- 26.1%; none of which were significantly different from that of the control. Four other extenders, 10% PG or 10% GLY with HBSS or KS, resulted in significantly lower fertilization rates. Use of a 4 mL cryopreservation volume did not exhibit a significant effect on fertilization rate or motility of post-thawed sperm compared to a 1.6 mL volume (P > 0.05); while the use of a dilution ratio of one part sperm with three parts ciyopreservation solution (1:3 v/v with sperm concentration of 0.51 +/- 0.11 x 10(10) cells/ml) had a significantly better preservation effect than using a ratio of 1:1 with sperm concentration of 1.02 +/- 0.21 x 10(10) cells/ml (P < 0.05). From these results, an optimized protocol for the cryopreservation of Atlantic halibut sperm using a volume as large as 4 mL has been established. (C) 2011 Elsevier Inc. All rights reserved.

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