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Construction and Characterization of a Novel Bacmid AcBac-Syn Based on a Synthesized Baculovirus Genome

文献类型： 外文期刊

作者： Shang, Yu 1 ; Hu, Hengrui 3 ; Wang, Xi 3 ; Wang, Hualin 3 ; Deng, Fei 3 ; Wang, Manli 3 ; Hu, Zhihong 4 ;

作者机构： 1.Hubei Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Prevent & Control Agents Anim Bacteriosis, Wuhan 430064, Peoples R China

2.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Prov Key Lab Anim Pathogen Microbiol, Wuhan 430064, Peoples R China

3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China

4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

关键词： Synthetic genome; Baculovirus; Autographa californica multiple nucleopolyhedrovirus (AcMNPV); Large DNA virus

期刊名称：VIROLOGICA SINICA （ 影响因子：4.327； 五年影响因子：4.08 ）

ISSN： 1674-0769

年卷期：

页码：

收录情况： SCI

摘要： Baculoviruses are large DNA viruses which have been widely used as expression vectors and biological insecticides. Homologous recombination and Bac-to-Bac system have been the main methods for manipulating the baculovirus genome. Recently, we generated a synthetic baculovirus AcMNPV-WIV-Syn1 which fully resembled its parental virus Autographa californica multiple nucleopolyhedrovirus (AcMNPV). Here, we report the modification of AcMNPV-WIV-Syn1 into a novel bacmid, AcBac-Syn, which can be used as a backbone for Bac-to-Bac system. To achieve this, a vector contained a LacZ:attTn7 and egfp cassette was constructed, and recombined with a linearized AcMNPV-WIV-Syn1 genome by transformation-associated recombination in yeast to generate bacmid AcBac-Syn. The bacmid was then transfected to insect cells and the rescued virus showed similar biological characteristics to the wild-type virus in terms of the kinetics of budded virus production, the morphology of occlusion bodies, and the oral infectivity in insect larvae. For demonstration, a red fluorescent protein gene Dsred was transposed into the attTn7 site by conventional Bac-to-Bac method, and the transfection and infection assays showed that AcBac-Syn can be readily used for foreign gene insertion and expression. AcBac-Syn has several advantages over the conventional AcMNPV bacmids, such as it contains an egfp reporter gene which facilitates visualization of virus propagation and titration; its DNA copy numbers could be induced to a higher level in E. coli; and the retaining of the native polyhedrin gene in the genome making it an attractive system for studying the functions of gene related to occlusion body assembly and oral infection.

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