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Proteomic profile of mouse oocytes after vitrification: A quantitative analysis based on 4D label-free technique

文献类型： 外文期刊

作者： Zhuan, Qingrui 1 ; Du, Xingzhu 1 ; Bai, Jiachen 2 ; Zhou, Dan 1 ; Luo, Yuwen 1 ; Liu, Hongyu 1 ; Sun, Wenquan 2 ; Wan, Pengcheng 3 ; Hou, Yunpeng 4 ; Li, Jun 1 ; Fu, Xiangwei 3 ;

作者机构： 1.China Agr Univ, Beijing Key Lab Anim Genet Improvem, Coll Anim Sci & Technol, Key Lab Anim Genet, Beijing, Peoples R China

2.Univ Shanghai Sci & Technol, Inst Biothermal Sci & Technol, Sch Med Instrument & Food Engn, Shanghai, Peoples R China

3.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, State Key Lab Sheep Genet Improvement & Healthy B, Shihhotze, Peoples R China

4.China Agr Univ, Coll Biol Sci, State Key Labs Agrobiotechnol, Beijing, Peoples R China

5.Hebei Med Univ, Hosp 1, Reprod Med Ctr, Dept Reprod Med, Shijiazhuang, Hebei, Peoples R China

关键词： Mice; Oocyte; Vitrification; Proteome; 4D label-free

期刊名称：THERIOGENOLOGY （ 影响因子：2.923； 五年影响因子：2.843 ）

ISSN： 0093-691X

年卷期： 2022 年 187 卷

页码：

收录情况： SCI

摘要： Mature oocyte cryopreservation represents an important trend for future fertility preservation, however, the relatively low efficiency has hampered its clinical application. Proteomic profiling is a method of choice for the exploration of the molecular mechanism underlying cryoinjuries. Here, a systematic comparison of protein expression between fresh and vitrified oocytes was performed based on the 4D label-free technique, an informative method with high sensitivity. Our results indicated that the oocyte survival rate was significantly reduced after vitrification. Proteomic results showed that 32 proteins were up-regulated, while 77 proteins were down-regulated in vitrified oocytes compared with the fresh counterparts. Gene Ontology (GO) functional analysis revealed that differentially expressed proteins (DEPs) were involved in metabolism, mitochondrial function, cytoskeleton and other cell functions. Moreover, proteins that participated in signaling transduction mechanisms were the largest category based on Clusters of Orthologous Groups of protein/EuKaryotic Orthologous Groups (COG/KOG) functional classification. In addition, over-expressed DEPs were enriched for "nucleus", "protein binding", "membrane", "cytoplasm" as well as mitochondrial function. Furthermore, we discovered that the DEPs were clustered in pyruvate metabolism, citric acid (TCA) cycle and glucose metabolism by Protein-Protein Interaction (PPI) network evaluation. In conclusion, our data demonstrate that vitrification induces multi-level damages in oocytes, the dynamic proteomic profiling will provide systematic insights into uncovering the mechanism underlying cryoinjuries. (C) 2022 Published by Elsevier Inc.

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