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Determination of Hydroxylated Polychlorinated Biphenyls in Mouse Tissues and Blood by Gas Chromatography/Mass Spectrometry

文献类型： 外文期刊

作者： Shi Yong-Fu 2 ; Lin Hong 2 ; Huang Dong-Mei 1 ; Gong Qian 2 ; Cai You-Qiong 1 ; Wang Yuan 1 ; Qian Bei-Lei 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Fishery Prod Qual Inspect & Test Ctr Shanghai, Minist Agr China, E China Sea Fishery Res Inst, Shanghai 200090, Peoples R China

2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

关键词： Hydroxylated polychlorinated biphenyls;Muscle;Blood;Gas chromatography/mass spectrometry

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2010 年 38 卷 9 期

页码：

收录情况： SCI

摘要： A method has been established to determine three hydroxylated metabolites of polychlorinated biphenyls(PCBs) in mouse muscle and blood by GC/MS. Target compouds were extracted by using hexane-ethyl acetate as extracting solvents(1:1, V/V). To obtain satisfactory peak shape and separation efficiency, sulfuric acid and alkaline stripping solution were used to remove the impurities. Derivatizing reagent was N,O-bis(trimethylsilyl)-trimethylchlorosilane (BSTFA-TMCS, 99:1, V/V). The target compounds were analyzed by GC/MS under SIM mode after silylation derivtization. The average recoveries of 3-hydroxy-2,2',4',5,5'-PeCB, 4-hydroxy-2',3,3',4',5'-PeCB and 4-hydroxy-2',3,3',5',6'-PeCB ranged from 73.2% to 113.2% spiked at 3 levels of 2, 5, 20 mu g/kg in muscle and blood. The range of relative standard deviations(RSD) in spiked samples was 1.5% - 10.4%. And the detection limit of the method was 2 mu g/kg. This established method has been successfully applied to the determination of hydroxylated metabolite in mouse tissues after intraperitoneal injection of 2,2',4',5,5'-PeCB at the dose of 10 mg/kg.

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