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Molecular characterization and expression analysis of interferon-gamma-inducible lysosomal thiol reductase (GILT) gene from pearl oyster Pinctada fucata

文献类型： 外文期刊

作者： Zhang, Dianchang 1 ; Pan, Dequan 1 ; Cui, Shuge 1 ; Su, Tianfeng 1 ; Qiu, Lihua 1 ; Zhu, Caiyan 1 ; Jiang, Shigui 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Aquaculture & Biotechnol Div, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

2.S China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

3.Jinan U

关键词： Pearl oyster;Pinctada fucata;Interferon-gamma-inducible lysosomal thiol reductase;Innate immunity

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2010 年 34 卷 9 期

页码：

收录情况： SCI

摘要： Interferon-gamma-inducible lysosomal thiol reductase (GILT) is an important thiol reductase, involved in class, MHC-restricted antigen processing by catalyzing disulfide bond reduction in mammals. Herein, we describe the identification and characterization of pearl oyster Pinctada fucata GILT (designated as poGILT). The poGILT cDNA was 1273 bp long and consisted of a 5'-untranslated region (UTR) of 24 bp. a 3'-UTR of 484 bp with two cytokine RNA instability motifs (ATTTA), and an open reading frame (ORF) of 765 bp encoding a polypeptide of 254 amino acids with an estimated molecular mass of 28.9 kDa and a theoretical isoelectric point of 7.4. The N-terminus of the poGILT was found to have a putative signal peptide with a cleavage site amino acid position at 19-20. SMART analysis showed that the poGILT contained a GILT active-site C(69)PDC(72) motif and a GILT signature motif C(115)QHGKEECIGNLIETC(130). Homology analysis of the deduced amino acid sequence of the poGILT with other known GILT sequences by Mat-GAT software revealed that the poGILT shared 42.9-67.3% similarity and 22.9-49.8% identity to the other known GILT sequences. The expression level of poGILT mRNA was higher in digestive gland, moderate in adductor muscle, gills, gonad, intestine and mantle, and lower in hemocytes. The poGILT mRNA expression was significantly up-regulated in gill and digestive gland after LPS or V. alginolyticus stimulation, respectively. These results suggested that the poGILT was a constitutively expressed acute-phase protein, the expression of which can be enhanced after LPS or V. algrinolyticus stimulation, perhaps involved in the innate immune response of pearl oyster. (C) 2010 Elsevier Ltd. All rights reserved.

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