文献类型： 外文期刊

作者： Wu, Di ¹ ; Wang, Liansheng ¹ ; Fan, Ze ¹ ; Li, Jinnan ¹ ; Tang, Shizhan ¹ ; Zhao, Chen ¹ ; Zhang, Haitao ⁶ ; Zheng, Xianhu ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China

2.Key Lab Aquat Anim Dis & Immune Technol Heilongjia, Harbin 150070, Peoples R China

3.Minist Agr & Rural Affairs Fishery Environm, Harbin 150070, Peoples R China

4.Aquat Prod Qual Supervis Inspect & Testing Ctr, Harbin 150070, Peoples R China

5.Minist Agr & Rural Affairs, Heilongjiang River Basin Fishery Ecol Environm Mon, Harbin 150010, Peoples R China

6.Guangdong Evergreen Feed Ind Co Ltd, Key Lab Aquat Livestock & Poultry Feed Sci & Techn, Minist Agr & Rural Affairs, Zhanjiang 524000, Peoples R China

7.Natl & Local Joint Engn Lab Freshwater Fish Breedi, Harbin 150070, Peoples R China

关键词： Copper; Hydrolysis peptides; Largemouth bass; Detoxification mechanism; Metabolomics; Lipid deposition

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 264 卷

页码：

收录情况： SCI

摘要： As a heavy metal, copper is toxic to aquatic organisms in water, causing oxidative stress and lipid deposition. However, there is currently no effective dietary strategy to prevent damage caused by copper exposure. Here, copper bioaccumulation, antioxidant enzymes, lipogenic enzymes, lipid metabolism-related gene expression levels and metabolic pathways were synthesized and evaluated in copper-exposed largemouth bass (Micropterus salmoides) after hydrolysis fish peptides (HFP) pretreatment. The results showed that supplementation with 1% (P < 0.05), 3% (P < 0.01) and 5% (P < 0.05) HFP significantly reduced the copper bioaccumulation in largemouth bass. Hydrolysis fish peptides supplementation significantly reduced the activities of total antioxidant capacity (P < 0.01) and catalase (P < 0.01) and the contents of glutathione (P < 0.01) and malondialdehyde (P < 0.05). Fatty acid synthetase concentration was significantly reduced in fish supplemented with 3% (P < 0.05) and 5% HFP (P < 0.05). Similarly, fish fed 3% (P < 0.05) and 5% (P < 0.01) HFP significantly reduced the glucose-6-phosphate dehydrogenase concentration. Serum metabolomics revealed that 85, 144 and 207 differential metabolites were obtained in fish supplemented with 1%, 3% and 5% HFP, respectively. The differential metabolites were mainly lipids and lipid-like molecules, which were associated with the lipid metabolism pathways. The expression levels of fatty acid synthase (P < 0.01), sterol regulatory element binding protein-1c (P < 0.05), liver X receptor (P < 0.001), peroxisome proliferator activated gamma (P < 0.01), apolipoprotein B (P < 0.001) and fatty acid-binding protein 1 (P < 0.01) were significantly down-regulated and the expression levels of carnitine palmitoyltransferase 1 alpha (P < 0.01), hormone-sensitive lipase (P < 0.001), apolipoprotein A 1 (P < 0.05) were significantly up-regulated in fish fed with 3% HFP. Additionally, supplementation with 3% (P < 0.01) and 5% (P < 0.001) HFP significantly up-regulated the expression level of B-cell lymphoma-2 with a dose-dependent effect. In conclusion, our study confirmed that HFP supplementation was closely associated with oxidative stress, enzymatic activities and related pathways of lipid metabolism, and apoptosis, and in general alleviated lipid deposition caused by copper exposure in largemouth bass.