Establishment and Application of Microsatellite Multiplex PCR System for Cheilinus undulatus
文献类型： 外文期刊
作者： Zhao, Fangcao 1 ; Guo, Liang 1 ; Zhang, Nan 1 ; Zhu, Kecheng 1 ; Yang, Jingwen 1 ; Liu, Baosuo 1 ; Guo, Huayang 1 ; Zhang, Dianchang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
2.Dalian Ocean Univ, Sch Fisheries & Life Sci, Dalian 116023, Peoples R China
3.Sanya Trop Fisheries Res Inst, Sanya 572019, Peoples R China
4.Guangdong Prov Engineer Technol Res Ctr Marine Bio, Guangzhou 510300, Peoples R China
关键词： Cheilinus undulatus; microsatellite; multiplex PCR; genetic diversity
期刊名称：JOURNAL OF MARINE SCIENCE AND ENGINEERING （ 影响因子：2.744； 五年影响因子：2.727 ）
ISSN：
年卷期： 2022 年 10 卷 12 期
页码：
收录情况： SCI
摘要： Cheilinus undulatus is a valuable seawater economic fish with tender meat, fresh taste, and high nutritional value; however, its population is rapidly declining because of massive fishing and habitat destruction. Assessing changes in genetic diversity and inbreeding levels is a very valuable monitoring tool, and multiplex PCR has the advantages of being time-efficient and economical. Here, we selected 12 pairs of polymorphic microsatellite loci, developed two multiplex PCR amplification systems based on these microsatellites, and used them to examine 30 C. undulatus specimens. The number of alleles (Na) for the 12 microsatellite markers ranged from 2 to 8. The effective allele number (Ne) ranged from 1.724 to 4.592. The expected heterozygosity (He) and observed heterozygosity (Ho) ranged from 0.420 to 0.782 and 0.100 to 0.900, respectively. The polymorphic information content (PIC) ranged from 0.422 to 0.746, with a mean value of 0.557. 5 loci deviated from Hardy-Weinberg equilibrium (HWE, p < 0.05 after Bonferroni correction). The multiplex PCR amplification system established in this study provides a basis for germplasm identification, genetic diversity analysis, and assessment of the effects of accretion and release of C. undulatus.
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