Establishment and Application of Microsatellite Multiplex PCR System for Cheilinus undulatus
文献类型： 外文期刊
作者： Zhao, Fangcao 1 ; Guo, Liang 1 ; Zhang, Nan 1 ; Zhu, Kecheng 1 ; Yang, Jingwen 1 ; Liu, Baosuo 1 ; Guo, Huayang 1 ; Zhang, Dianchang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
2.Dalian Ocean Univ, Sch Fisheries & Life Sci, Dalian 116023, Peoples R China
3.Sanya Trop Fisheries Res Inst, Sanya 572019, Peoples R China
4.Guangdong Prov Engineer Technol Res Ctr Marine Bio, Guangzhou 510300, Peoples R China
关键词： Cheilinus undulatus; microsatellite; multiplex PCR; genetic diversity
期刊名称：JOURNAL OF MARINE SCIENCE AND ENGINEERING （ 影响因子：2.744； 五年影响因子：2.727 ）
ISSN：
年卷期： 2022 年 10 卷 12 期
页码：
收录情况： SCI
摘要： Cheilinus undulatus is a valuable seawater economic fish with tender meat, fresh taste, and high nutritional value; however, its population is rapidly declining because of massive fishing and habitat destruction. Assessing changes in genetic diversity and inbreeding levels is a very valuable monitoring tool, and multiplex PCR has the advantages of being time-efficient and economical. Here, we selected 12 pairs of polymorphic microsatellite loci, developed two multiplex PCR amplification systems based on these microsatellites, and used them to examine 30 C. undulatus specimens. The number of alleles (Na) for the 12 microsatellite markers ranged from 2 to 8. The effective allele number (Ne) ranged from 1.724 to 4.592. The expected heterozygosity (He) and observed heterozygosity (Ho) ranged from 0.420 to 0.782 and 0.100 to 0.900, respectively. The polymorphic information content (PIC) ranged from 0.422 to 0.746, with a mean value of 0.557. 5 loci deviated from Hardy-Weinberg equilibrium (HWE, p < 0.05 after Bonferroni correction). The multiplex PCR amplification system established in this study provides a basis for germplasm identification, genetic diversity analysis, and assessment of the effects of accretion and release of C. undulatus.
- 相关文献
作者其他论文 更多>>
-
Deciphering Trachinotus ovatus resistance to Streptococcus agalactiae:GWAS :GWAS identification of key SNPs and candidate genes
作者：Sun, Yi-Yao;Gao, Jie;Liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Zhu, Ke-Cheng;Xian, Lin;Zhang, Dian-Chang;Sun, Yi-Yao;Liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Zhu, Ke-Cheng;Xian, Lin;Zhang, Dian-Chang;Liu, Bao-Suo;Zhang, Nan;Zhu, Ke-Cheng;Zhang, Dian-Chang;Gao, Jie
关键词：GWAS; Trachinotus ovatus; Streptococcus agalactiae; SNP
-
Functional Characterization of Galectin-8 from Golden Pompano Trachinotus ovatus Reveals Its Broad-Spectrum Antimicrobial Activity
作者：Pan, Jin-Min;Gao, Jie;Liu, Ming Jian;Zhu, Ke-Cheng;Guo, Hua-Yang;Liu, Bao-Suo;Zhang, Nan;Zhang, Dian-Chang;Zhu, Ke-Cheng;Liu, Bao-Suo;Zhang, Nan;Zhang, Dian-Chang;Zhu, Ke-Cheng;Guo, Hua-Yang;Liu, Bao-Suo;Zhang, Nan;Zhang, Dian-Chang;Pan, Jin-Min;Gao, Jie
关键词：
Galectin-8 ; Antibacterial activity;Trachinotus ovatus ;Streptococcus agalactiae -
Identification, genome-wide sequencing and analysis of drug resistance genes in a novel Vibrio harveyi strain isolated from yellowfin seabream
作者：Huang, Zhihong;Guo, Min;Peng, Dikuang;Lai, Chuchu;Dan, Xueming;Yang, Min;Lee, Xuezhu;Huang, Zhihong;Guo, Min;Peng, Dikuang;Lai, Chuchu;Dan, Xueming;Yang, Min;Lee, Xuezhu;Huang, Zhihong;Dan, Xueming;Yang, Min;Lee, Xuezhu;Zhu, Kecheng
关键词：Vibrio harveyi; Yellowfin seabream; Bacterial genome sequencing; Antibiotic resistance
-
Chromosome-level genome assembly and annotation of the White-spotted spinefoot Siganus canaliculatus
作者：Huang, Xiaolin;Xian, Lin;Yang, Yukai;Zhang, Dianchang;Huang, Xiaolin;Xian, Lin;Yang, Yukai;Zhang, Dianchang;Huang, Xiaolin;Yang, Yukai;Zhang, Dianchang;Lu, Yanke;Zhang, Hui;Huang, Shiting;Wang, Lei;Li, Chao;Xian, Lin
关键词：
-
Acute alkalinity stress induces functional damage and alters immune metabolic pathways in the gill tissue of spotted scat (Scatophagus argus)
作者：Yang, Lei;Han, Tong;Duan, Cunyu;Chen, Yu;Li, Xiaolong;Shi, Gang;Zhu, Chunhua;Li, Guangli;Tian, Changxu;Zhu, Kecheng;Abudu, Adili
关键词：Alkalinity stress; Scatophagus argus; Histology; Biochemical; Transcriptome
-
Gut microbiome composition likely affects the growth of Trachinotus ovatus by influencing the host's metabolism
作者：Wu, Chen-Xu;Zhang, Dian-Chang;Wu, Chen-Xu;Liu, Bao-Suo;Guo, Hua-Yang;Zhu, Teng-Fei;Xian, Lin;Zhang, Nan;Zhu, Ke-Cheng;Zhang, Dian-Chang;Guo, Hua-Yang;Zhang, Nan;Zhu, Ke-Cheng;Zhang, Dian-Chang;Liu, Bao-Suo;Zhang, Nan;Zhu, Ke-Cheng;Zhang, Dian-Chang;Liu, Bao-Suo;Zhang, Dian-Chang
关键词：Trachinotus ovatus; Growth differences; Gut microbiota; Interspecies interactions
-
Identification and functional analysis of glutathione S-transferase gene family in Trachinotus ovatus: Transcriptomic response to environmental and pathogenic stress
作者：Zhou, Jia-Mei;Zhang, Tian-Yue;Zhou, Jia-Mei;Zhu, Teng-Fei;Guo, Hua-Yang;Xian, Lin;Liu, Bao-Suo;Zhang, Nan;Zhang, Tian-Yue;Zhu, Ke-Cheng;Zhang, Dian-Chang;Zhu, Teng-Fei;Guo, Hua-Yang;Xian, Lin;Zhang, Nan;Zhu, Ke-Cheng;Zhang, Dian-Chang;Liu, Bao-Suo;Zhang, Nan;Zhu, Ke-Cheng;Zhang, Dian-Chang;Liu, Bao-Suo
关键词：Glutathione S-transferase; Trachinotus ovatus; Hypoxia stress; Pathogenic stress
